TABLE 1.

Microorganisms and plasmids

MicroorganismRelated genotype, phenotype, or featureSource/reference
Strains
    Corynebacterium ammoniagenesATCC 6872, wild type, nrdE+Institute's culture collection
    Strain CH31Thermosensitive mutant of ATCC 6872, nrdE(Ts)18
    Escherichia coli XL1-BlueendA1 gyrA96 hsdR17 (rK mK+) recA1 relA1 supE44 thi-1 F′(proAB lacIqZΔM15 Tn10)Stratagene
Plasmids
    pUC18Ampr, cloning vector45
    pUCEF6872pUC18 with a 5.2-kb XmaI fragment from C. ammoniagenes ATCC 6872This work
    pUCEFCH31pUC18 with a 5.2-kb XmaI fragment from C. ammoniagenes CH31This work
    pXMJ19Cmr, C. glutamicum/E. coli shuttle vector13
    pXE6872pXMJ19 containing the nrdE+ gene from C. ammoniagenes ATCC 6878 (accession no. AY769914) cloned by PCRThis work
    pXECH31pXMJ19 containing the nrdE(Ts) gene from C. ammoniagenes CH31 (accession no. AY788949) cloned by PCRThis work