TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescription or genotypeaReference
B. cenocepacia strains
    K56-2Epidemic cystic fibrosis respiratory isolate, genomovar III, cblA+, BCESM+28
    K56-I2cepI::tmp derivative of K56-2; Tpr28
    K56-R2cepR::Tn5-OT182 derivative of K56-2; Tcr28
    K56-2 cciIcciI::tp derivative of K56-25
    K56-2 cciRcciR::tp derivative of K56-235
    K56-2 cepI cciIaΔcepI cciI::tp derivative of K56-2 with a spontaneous mutation affecting ornibactin synthesis35
    K56-2 cepI cciIbΔcepI cciI::tp derivative of K56-235
    K56-2 cepR cciIRcepR::tp ΔcciR derivative of K56-235
Plasmids
    pHKT1pBBR1MCS-3 containing 1.6-kbp KpnI/SstI gfp fragment from pASV2; Tcr54
    pHKT2pBBR1Tp containing 1.6-kbp KpnI/XbaI gfp fragment from pASV2; Tpr54
    pUCP28TBroad-host-range vector; Tpr48
    pUCP26Broad-host-range vector; Tcr62
    pSLA3.2pUCP28T with 3.2-kb SphI fragment containing cepI; Tpr28
    pSLR100pUCP28T with 1.65-kb KpnI-SphI fragment from pSLA3.2 containing cepR; Tpr28
    pSLS225pUCP26 with 1.5-kb SphI-KpnI fragment from pSLA3.2 containing cepI; Tcr29
    pRM4.3pCR2.1TOPO with 4.3-kb XhoI fragment containing cciIR; Kmr35
    pRM164pUCP26 with 850-bp PCR amplified cciI fragment; Tcr35
    pRM165pUCP26 with 1.1-kb NcoI-ApaLI fragment from pRM4.3; TcrThis paper
    pRM166pUCP26 with 1.6-kb SphI-KpnI fragment from pSLA3.2; TcrThis paper
  • a cblA, cable pilus gene; BCESM, Burkholderia cepacia epidemic strain marker; Tp, trimethoprim; Tc, tetracycline; Ap, ampicillin; Km, kanamycin.