TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescription or genotypeaReference
B. cenocepacia strains
    K56-2Epidemic cystic fibrosis respiratory isolate, genomovar III, cblA+, BCESM+ 28
    K56-I2 cepI::tmp derivative of K56-2; Tpr 28
    K56-R2 cepR::Tn5-OT182 derivative of K56-2; Tcr 28
    K56-2 cciI cciI::tp derivative of K56-2 5
    K56-2 cciR cciR::tp derivative of K56-2 35
    K56-2 cepI cciIaΔcepI cciI::tp derivative of K56-2 with a spontaneous mutation affecting ornibactin synthesis 35
    K56-2 cepI cciIbΔcepI cciI::tp derivative of K56-2 35
    K56-2 cepR cciIR cepR::tp ΔcciR derivative of K56-2 35
Plasmids
    pHKT1pBBR1MCS-3 containing 1.6-kbp KpnI/SstI gfp fragment from pASV2; Tcr 54
    pHKT2pBBR1Tp containing 1.6-kbp KpnI/XbaI gfp fragment from pASV2; Tpr 54
    pUCP28TBroad-host-range vector; Tpr 48
    pUCP26Broad-host-range vector; Tcr 62
    pSLA3.2pUCP28T with 3.2-kb SphI fragment containing cepI; Tpr 28
    pSLR100pUCP28T with 1.65-kb KpnI-SphI fragment from pSLA3.2 containing cepR; Tpr 28
    pSLS225pUCP26 with 1.5-kb SphI-KpnI fragment from pSLA3.2 containing cepI; Tcr 29
    pRM4.3pCR2.1TOPO with 4.3-kb XhoI fragment containing cciIR; Kmr 35
    pRM164pUCP26 with 850-bp PCR amplified cciI fragment; Tcr 35
    pRM165pUCP26 with 1.1-kb NcoI-ApaLI fragment from pRM4.3; TcrThis paper
    pRM166pUCP26 with 1.6-kb SphI-KpnI fragment from pSLA3.2; TcrThis paper
  • a cblA, cable pilus gene; BCESM, Burkholderia cepacia epidemic strain marker; Tp, trimethoprim; Tc, tetracycline; Ap, ampicillin; Km, kanamycin.