TABLE 2.

Amplification and sequencing primers for rpoA, recA, and pyrH

Gene productPrimerSequence (5′→3′)Position
Uridylate kinase (pyrH; 750 nt)pyrH-02-RGTRAABGCNGMYARRTCCA599
pyrH-04-FATGASNACBAAYCCWAAACC1
RNA polymerase alpha chain (rpoA; 1,000 nt)rpoA-01-FATGCAGGGTTCTGTDACAG1
rpoA-03-RGHGGCCARTTTTCHARRCGC951
rpoA-05-FGCAGCDCGTGTWGARCARCG568
rpoA-06-RCGYTGYTCWACACGHGCTGC568
RecA protein (recA; 1,300 nt)recA-01-FTGARAARCARTTYGGTAAAGG222
recA-02-RTCRCCNTTRTAGCTRTACC1040
recA-03-FTYGGBGTGATGTTYGGTAACC767
recA-04-RGGGTTACCRAACATCACVCC769