TABLE 5

Strains used in this studya

StrainRelevant genotypeResult(s) of mutationCharacteristic(s)Source or reference
MG1655F λ ΔilvG rfb-50 rph-1ATCC 700926
CARsoxR (G4275900T); 95-kb deletion (positions 3623620–3718650)Predicted SoxR missense mutation D137Y; 1 large chromosomal deletion, Δ(yhhJ-mokA)MG1655 10-day carvacrol selection11
CITgyrB (−GGT3877671); marR (+A1617171); intergenic eptB-yhjX (A3708613C)GyrB in-frame ΔG157, MarR E10 frameshiftMG1655 10-day citral selection11
LIMacrR (+C485340); 4-kb deletion (positions 1972710–1976520); 11-kb deletion (positions 2800250–2811150)ArcR F119 frameshift; 2 large chromosomal deletions, Δ(cheA-insA) and Δ(nrdE-enrB)MG1655 10-day (+)-limonene oxide selection11
CAG12156MG1655 uvrC279::Tn1060
CAG12164MG1655 malF3089::Tn1060
CAG18592MG1655 zie-3163::Tn10Kan60
BC010soxR rev. WT (MG1655)CAR, soxR rev. WT, Tetr nearby; P1(CAG12164) × CARThis study
BC020gyrB rev. WT (MG1655)CIT, gyrB rev. WT, Kanr nearby; P1(CAG18592) × CITThis study
BC021gyrB (−GGT3877671)GyrB in-frame ΔG157CIT, gyrB Kanr nearby; P1(CAG18592) × CITThis study
BC022gyrB (−GGT3877671)GyrB in-frame ΔG157MG1655, gyrB (−GGT157) Kanr nearby; P1(BC021) × MG1655This study
BC0304-kb deletion rev. WT (MG1655)LIM, 4-kb deletion rev. WT, Tetr nearby; P1(CAG12156) × LIMThis study
BC0314-kb deletion (positions 1972710–1976520)1 large chromosomal deletion, Δ(cheA-insA)LIM, 4-kb deletion (positions 1972710–1976520) Tetr nearby; P1(CAG12156) × LIMThis study
BC0324-kb deletion (positions 1972710–1976520)1 large chromosomal deletion, Δ(cheA-insA)MG1655, 4-kb deletion (positions 1972710–1976520) Tetr nearby; P1(BC031) × MG1655This study
  • a rev., reverted to.