TABLE 2.

Substrate specificity of purified YjgB-His with respect to purified reduced muropeptides

PeakaMuropeptideb% Digested muropeptidec
1DS-Tri5.4
2DS-Tetra70.5
3DS-Tri-D2.1
4DS-Tri*-N1.7
5DS-Tri-N3.3
6DS-Tetra-D84.2
7DS-Penta-D98.3
8DS-Tetra-N92.2
9DS-Penta-N92.5
  • a Peak numbers refer to chromatogram shown in Fig. 3.

  • b DS-Tri, disaccharide tripeptide (l-Ala-γ-D-Gln-l-Lys); DS-Tri*, disaccharide tripeptide (l-Ala-γ-d-Glu-l-Lys); DS-Tetra, disaccharide tetrapeptide (l-Ala-γ-d-Gln-l-Lys-d-Ala); DS-Penta, disaccharide pentapeptide disaccharide (l-Ala-γ-d-Gln-l-Lys-d-Ala-d-Ala); disaccharide, GlcNAc-MurNAc (GlcNAc, N-acetylglucosamine, MurNAc, N-acetylmuramic acid); D, d-Asp; N, d-Asn.

  • c The percentage of digested muropeptide was calculated as the ratio of the DS-di peak area to the sum of the peak areas of the nondigested muropeptide substrate and the DS-di product.