TABLE 1.

Strains and plasmids used in this study

Strain or plasmidCharacteristicsReference(s) or source
Strains
    Lactococcus lactis NZ9000nisRK+23
    L. lactis PA1001 (derived from NZ9000)nisRK+ ΔacmA3
    L. lactis NZ9700nisABTCIPRKEFG23
    L. lactis LL108(pOri 280)Emr Cmr23,26
    Micrococcus flavus B42317
    Streptococcus thermophilus ST11ΔlacS31
    Staphylococcus epidermidis K737
    Bacillus subtilis 168fenABCDE Δsfp Δsrf25
    Leuconostoc mesenteroides 20343DSMZ
    Lactobacillus johnsonii 10533DSMZ
    Pediococcus pentosaceus 20336DSMZ
Plasmids
    pIL253-derived plasmids35
    pIL3BTCnisBTC32
    pNZ8048-derived plasmids24
    pNZnisA-E3Nisin A18
    pNGnisPnisP18
    pTP-ITRICKLeader ITRICK32
    pTP-KFIEncodes I4K S5F L6I nisin AThis study