TABLE 1.

Data summary of Q-PCR and PLFA groups for stimulated and control column sediment samples collected from sample ports 1 to 8

ParameterValue for sediment samples from:Value for sediment samples from:
Stimulated columnControl column
EtOH 1EtOH 2EtOH 3EtOH 4EtOH 5EtOH 6EtOH 7EtOH 8AvgSDC1C2C3C4C5C6C7C8AvgSD
PLFA
    Viable biomass (cells/g sediment)4.28E+086.46E+084.80E+086.49E+086.99E+085.18E+088.16E+084.53E+085.86E+081.28E+084.36E+064.39E+068.98E+061.48E+076.59E+064.43E+061.29E+072.08E+067.32E+064.24E+06
    Community structure (% total PLFA)
        Terminally branched saturates14.616.020.022.217.321.420.420.819.12.5717.616.414.514.015.214.310.310.414.12.43
        Branched monounsaturates3.163.833.945.303.763.864.293.884.000.573.832.283.282.922.522.761.831.602.630.69
        Polyunsaturates0.2420.2300.4480.3570.4230.4340.5100.7880.430.161.751.051.671.591.541.391.250.721.370.33
        Midchain-branched saturates3.155.154.694.826.807.568.928.956.261.999.528.659.159.1111.712.310.59.4910.01.23
        Normal saturates16.621.318.918.819.920.320.620.619.61.3922.226.022.823.723.923.827.128.524.72.07
        Monounsaturates61.953.452.048.451.846.345.144.950.55.3145.145.748.648.745.245.449.049.347.11.81
        Hydroxy0.2480.0620.0540.1160.1110.1300.1290.1500.130.060.000.000.000.000.000.000.000.000.000.00
        Shannon-Weiner diversity index2.592.822.882.882.933.043.043.142.910.163.092.883.032.973.043.092.792.652.940.15
    Metabolic status (ratio)
        cy17:0/16:1w7c0.0840.0510.0600.0580.0530.0740.0470.0830.0640.0140.3770.3290.5490.3940.4880.4110.4960.6760.4650.104
        cy19:0/18:17c0.0570.0800.0940.1030.1130.0960.1810.1290.1070.0350.2600.2670.2930.2310.2560.2620.2240.2130.2510.024
        Total cyc/monoa0.1410.1310.1540.1610.1660.1690.2280.2120.1700.0310.6370.5960.8420.6250.7440.6730.7200.8900.7160.098
        16:1w7t/16:1w7c0.0700.0700.0940.1140.0990.1220.1020.1550.1030.0260.0630.0540.0800.0650.0620.0690.0540.0630.0640.008
        18:1w7t/18:1w7c0.0340.0360.0330.0330.0330.0350.0640.0750.0430.0160.0540.0380.0760.0630.0620.0660.0360.0720.0580.014
        Total trans/cisb0.1040.1060.1270.1460.1310.1570.1650.2300.1460.0380.1170.0920.1560.1270.1240.1350.0900.1340.1220.021
        i15:0/a15:00.8310.7670.7250.4530.6170.5400.6700.8380.6800.1291.211.371.281.471.391.381.451.381.3680.081
        i17:0/a17:00.2450.1980.1930.1810.1720.2290.2490.2950.2200.0390.6950.8590.6480.7360.7020.7130.7200.9450.7520.092
        Total iso/anteisoc1.080.9650.9170.6350.7890.7690.9191.130.9000.1541.912.231.922.212.092.102.172.332.1200.138
        DMAs (pmol/g sediment)2063433973264795311,01261248823116.51.7928.218.315.98.6514.98.19
        UQ/MQ ratio2.532.142.364.074.333.683.190.873.492.772.972.452.121.681.380.3902.160.926
Q-PCR (copies/g sediment)d
    Eubacterial 16S rRNA1.23E+093.94E+082.08E+091.88E+093.11E+092.00E+092.65E+092.07E+091.93E+097.76E+085.00E+075.83E+075.83E+076.91E+062.11E+071.59E+078.22E+063.68E+063.08E+072.71E+07
    nirS2.89E+083.08E+073.06E+083.55E+084.35E+084.53E+085.99E+081.17E+094.55E+083.10E+081.02E+078.20E+067.69E+061.17E+062.80E+062.44E+069.57E+061.65E+065.46E+063.55E+06
    nirK9.31E+075.56E+071.85E+082.07E+082.20E+082.46E+082.45E+083.97E+082.06E+089.72E+074.48E+069.99E+061.80E+075.12E+069.35E+064.43E+062.11E+04ND6.42E+065.55E+06
    Deltaproteobacteria3.88E+05ND4.67E+073.46E+081.78E+082.22E+081.49E+083.52E+081.62E+081.32E+083.08E+073.25E+064.05E+062.44E+045.42E+055.42E+052.44E+042.36E+034.91E+069.90E+06
    Geobacteraceae108*ND205*642*4.64E+041.88E+044.82E+031.98E+041.13E+041.54E+04<100<100<100NDND<100112*ND
    MethanogensNDNDNDNDNDNDNDND6.61E+055.56E+062.25E+054.18E+052.64E+053.27E+052.64E+053.22E+051.01E+061.73E+06
    Methylotrophs6.24E+06ND5.54E+051.60E+051.35E+051.31E+051.72E+054.34E+059.78E+052.00E+063.72E+052.15E+075.53E+052.82E+051.90E+055.14E+053.63E+05ND2.97E+067.00E+06
  • a cyc/mono, ratio of cyclopropane to monoenoic precursor fatty acids.

  • b trans/cis, ratio of monounsaturated trans to cis isomers.

  • c iso/anteiso, ratio of iso- to anteiso-saturated fatty acids.

  • d *, estimated value; <100, below detection limit; ND, not detected (sample not processed).