TABLE 1.

Primers utilized for probe generation in Northern blot analysis

ORFDescriptionPrimer
OrientationaSequence (5′ to 3′)b
LBA0197ABC transporter, oligopeptide-binding protein OppA1FGCAGCATGTAGTAGTAATAA
RCAGAATCACGTAATGTGTAA
LBA1300Oligopeptide ABC transporter, substrate-binding protein OppA2FATGCAATAGCTTGACGAAGA
RATGCAATATGGTGCTGAATC
LBA1524Two-component sensor histidine kinaseFGATCTCTAGA-CAGCGCTCTAGCA
RGATCAGATCT-TCGGCCAATGTG
LBA1525Two-component system regulatorFGATCTCTAGA-CACGAACCGTCTT
RGATCAGATCT-TTGGCTCGATTTG
LBA0698Glyceraldehyde-3-phosphate dehydrogenaseFTCGTAGTTGACGGTAAGAAG
RACCTGCAGTAGTTACCATAG
LBA1075Malolactic enzymeFGTTGTTACAGACGGTGAAGG
RTAATGCACGACCATCAGTCC
LBA1196RNA polymerase sigma factor RpoDFGATCTCTAGA-TTCCGCTTCTTACT
RGATCAGATCT-ATCTGACGAATACG
  • a F, forward; R, reverse.

  • b Dashes indicate the introduction of restriction enzyme sites.