TABLE 3.

S. meliloti genes significantly repressed under iron-limiting conditions and in the rirA mutanta

Open reading frameGene and/or descriptionExpression ratiob
VMMTY mediumΔrirA
SMa0249Conserved hypothetical protein−1.12−1.06−0.07
SMa0252Conserved hypothetical protein−2.32−1.09−0.40
SMa1289Hypothetical protein−1.68−1.53−0.10
SMa2032Putative nonheme chloroperoxidase−1.70−1.92−1.11
SMb20293Hypothetical protein−1.47−1.21−0.03
SMb20397Putative oxidoreductase protein−2.85−1.69−0.47
SMb20649nadE1, putative NH3-dependent NAD+ synthetase protein−1.09−1.62−0.02
SMb20650Putative long-chain fatty acid coenzyme A ligase protein−1.18−1.71−0.02
SMb20651Hypothetical protein−2.30−1.960.10
SMb20764phnL, putative phosphonate uptake ABC transporter ATP binding protein−2.76−2.31−0.29
SMb20986narB, putative nitrate reductase large-subunit protein−1.61−1.390.39
SMb21170Putative guanylate kinase−1.08−1.41−0.25
SMb21174phoT, phosphate uptake ABC transporter permease protein−1.07−1.75−0.11
SMb21175phoE, phosphate uptake ABC transporter permease protein−1.70−1.90−0.31
SMb21177phoC, phosphate uptake ABC transporter ATP binding protein−1.36−1.31−0.15
SMc00158Hypothetical protein−1.41−2.25−0.22
SMc00159Hypothetical protein−1.70−2.120.12
SMc00283Putative transcription regulator protein−3.82−3.05−0.64
SMc00335rpsA, 30S ribosomal protein s1−0.44−1.14−1.05
SMc00565rplI, probable 50S ribosomal protein 19−0.63−1.12−1.05
SMc00578Hypothetical protein−0.47−2.05−1.20
SMc00765mcpZ, probable methyl-accepting chemotaxis protein−3.68−3.29−0.23
SMc00887Hypothetical protein−3.35−3.43−0.50
SMc00888Hypothetical protein−3.08−3.01−0.08
SMc00975mcpU, probable chemoreceptor (methyl-accepting chemotaxis) transmembrane protein−4.11−4.14−0.81
SMc00986Hypothetical protein−1.83−1.79−0.45
SMc00998Conserved hypothetical signal peptide protein−1.63−1.190.08
SMc01104mcpX, probable chemoreceptor (methyl-accepting chemotaxis) transmembrane protein−2.98−3.67−0.36
SMc01160Putative transcriptional regulator protein−2.51−0.47−1.17
SMc01319rplJ, probable 50S ribosomal protein−1.33−1.10−1.51
SMc01326tufB, probable elongation factor Tu protein−1.04−0.42−1.25
SMc01469mcpW, probable methyl-accepting chemotaxis transmembrane protein−4.38−3.41−0.63
SMc01842Putative methyltransferase transcription regulator protein−0.49−2.05−1.00
SMc01847Putative methyltransferase protein−1.40−1.25−0.92
SMc02392Hypothetical protein−1.39−1.81−0.76
SMc02634Hypothetical transmembrane protein−2.60−2.12−0.91
SMc03004mcpE, putative chemoreceptor (methyl-accepting chemotaxis protein)−1.36−1.44−0.57
SMc03005Conserved hypothetical protein−1.21−1.85−0.30
SMc03007cheA, chemotaxis protein (sensory transduction histidine kinase)−2.80−3.04−0.57
SMc03008cheW1, chemotaxis protein−2.04−2.29−0.37
SMc03009cheR, chemotaxis protein methyltransferase−4.21−3.68−0.43
SMc03012cheD, chemotaxis protein−2.18−2.00−0.63
SMc03013Conserved hypothetical protein−2.07−2.03−0.52
SMc03017Conserved hypothetical protein−1.61−1.80−0.84
SMc03021fliM, flagellar motor switch transmembrane protein−2.41−2.83−0.71
SMc03022motA, chemotaxis (motility protein A)−2.66−2.81−0.80
SMc03023Conserved hypothetical protein−2.42−2.69−0.76
SMc03024flgF, flagellar basal body rod protein−3.14−3.36−1.00
SMc03027flgB, flagellar basal body rod protein−3.25−3.50−0.84
SMc03028flgC, flagellar basal body rod protein−1.35−1.74−0.59
SMc03029fliE, flagellar hook basal body complex protein−3.20−2.72−0.79
SMc03030flgG, flagellar basal body rod protein−3.35−3.30−0.89
SMc03031flgA, flagellar precursor transmembrane protein−1.16−1.88−0.69
SMc03034flgH, flagellar 1 ring protein precursor−1.62−1.97−0.56
SMc03035fliL, flagellar transmembrane protein−2.66−3.11−1.19
SMc03037flaA, flagellin protein−3.95−4.37−0.11
SMc03038flaB, flagellin protein−3.97−3.76−0.42
SMc03040flaC, flagellin protein−2.28−2.36−0.45
SMc03042motB, chemotaxis protein−1.84−2.00−0.83
SMc03043motC, chemotaxis protein precursor−1.92−2.49−0.96
SMc03044motD, chemotaxis protein−2.97−3.04−0.82
SMc03045Hypothetical transmembrane protein−4.50−1.41
SMc03047flgE, flagellar hook protein−1.66−1.61−0.50
SMc03048flgK, putative flagellar hook-associated protein−2.50−1.90−0.81
SMc03049flgL, putative flagellar hook-associated protein−3.14−2.25−0.85
SMc03051flbT, putative flagellin synthesis repressor protein−2.21−1.90−0.68
SMc03052flgD, putative basal body rod modification protein−1.94−1.71−0.64
SMc03057Conserved hypothetical transmembrane protein−1.57−2.56−0.74
SMc03071Hypothetical protein−1.68−2.06−0.56
SMc03072Conserved hypothetical protein−3.35−2.48−0.62
SMc03242Probable GTP binding protein−0.21−1.10−1.61
SMc03857ffh, probable signal recognition particle protein−0.84−1.16−1.64
SMc04007Conserved hypothetical protein−1.18−0.86−1.19
SMc04009Conserved hypothetical protein−0.85−1.03−1.14
SMc04059Hypothetical protein−2.86−3.23−0.66
SMc04300afuC, probable iron ABC transporter ATPase−0.75−1.40−1.39
SMc04317afuA, probable iron ABC transporter periplasmic binding protein−1.52−1.55−0.90
  • a Genes were considered differentially expressed when according to t-statistics the P value was <0.05 with a repression level of >two fold. Only genes differentially expressed during at least two sets of microarray experiments were considered.

  • b The expression ratios are expressed as log2 values.