TABLE 1.

Set of PCR primers used for the development of specific amplification protocols and DGGE fingerprinting of seven different phylogenetic groups of bacteria

DesignationaGroupPrimer sequencebReference(s)
GC-517fα-Proteobacteria5′-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCCGTGCCAGCAGCCGCGG-3′31
Alf968r5′-GGTAAGGTTCTGCGCGTT-3′41
Beta680fβ-Proteobacteria5′-CRCGTGTAGCAGTGA-3′44
GC-1055r5′-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCCAGCTGACGACAGCCAT-3′3
GC-517fActinobacteria5′-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCCGTGCCAGCAGCCGCGG-3′31
AB1165r5′-ACCTTCCTCCGAGTTRAC-3′33
8fPlanctomyces5′-AGAGTTTGATCCTGGCTCAG-3′31
PLA886r5′-GCCTTGCGACCATACTCCC-3′42
GC-LGC354fFirmicutes5′-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCCGCAGTAGGGAATCTTCSR-3′36
907r5′-CCGTCAATTCCTTTGAGTTT-3′40
GC-CFB319fFlavobacteria-Cytophaga5′-CGCCCGCCGCCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCCGTACTGAGACACGGACCA-3′35, 26
907r5′-CCGTCAATTCCTTTGAGTTT-3′40
GC-341fAll groups5′-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCCCTCCTACGGGAGGCAGCAG-3′40
907r5′-CCGTCAATTCCTTTGAGTTT-3′40
  • a f, forward; r, reverse; GC, GC clamp.

  • b Degenerated oligonucleotides are indicated according to IUPAC-IUB notation: R = A/G; S = C/G.