TABLE 2.

S. meliloti genes significantly induced under iron-limiting conditions and in the rirA mutanta

Open reading frameGene and/or descriptionExpression ratiob
VMMTY mediumΔrirA
SMa0002fdoG, probable FdoG formate dehydrogenase-O alpha subunit−0.422.131.05
SMa0142Possible protease1.601.291.08
SMa0172Conserved hypothetical protein1.271.170.96
SMa0312Hypothetical protein2.431.742.09
SMa0314Hypothetical protein1.240.921.21
SMa0316Conserved hypothetical protein2.310.901.56
SMa0320Putative dehydrogenase1.810.491.68
SMa0612fixN3, cytochrome c oxidase subunit 11.971.611.26
SMa0617fixP3, cytochrome c oxidase membrane-anchored subunit1.271.051.62
SMa0621fixI2, E1-E2-type cation ATPase1.000.521.11
SMa0994Hypothetical protein1.371.921.08
SMa1461Putative muconate cycloisomerase1.130.661.47
SMa1683Putative arylsulfatase2.571.021.74
SMa1686Putative two-component response regulator1.051.090.54
SMa1745Putative iron ABC transporter permease1.090.511.39
SMa1746Putative iron ABC transporter periplasmic binding protein1.981.962.41
SMa1749Putative transcriptional regulator0.471.161.23
SMa1860Putative ABC transporter periplasmic binding protein2.131.051.02
SMa1985Hypothetical protein1.090.831.56
SMa2259Hypothetical protein1.250.771.36
SMa2294mrcA2, probable penicillin binding protein1.351.47
SMa2337rhtX, rhizobactin 1021 transporter4.953.394.96
SMa2339Putative siderophore biosynthesis protein5.093.176.28
SMa2400rhbA, rhizobactin 1021 biosynthesis protein7.865.207.50
SMa2402rhbB, rhizobactin 1021 biosynthesis protein5.814.855.78
SMa2404rhbC, rhizobactin 1021 biosynthesis protein4.342.024.74
SMa2406rhbD, rhizobactin 1021 biosynthesis protein5.213.115.40
SMa2408rhbE, rhizobactin 1021 biosynthesis protein7.194.666.70
SMa2410rhbF, rhizobactin 1021 biosynthesis protein6.384.476.38
SMa2412rhrA, transcriptional activator1.401.752.42
SMa2414rhtA, rhizobactin 1021 receptor4.723.964.27
SMb20022Conserved hypothetical protein1.221.231.36
SMb20072Putative rhizopine binding protein1.063.460.37
SMb20099Putative trehalose synthase protein1.811.171.18
SMb20127Hypothetical protein1.360.562.88
SMb20203cbbR, probable transcriptional regulator protein1.070.631.38
SMb20545Hypothetical protein1.190.151.31
SMb20600Hypothetical protein1.731.430.75
SMb20755pccB, putative propionyl-coenzyme A carboxylase beta chain protein1.182.070.24
SMb20757bhbA, methylmalonyl-coenzyme A mutase protein1.402.070.52
SMb20924abfA, putative alpha-L-arabinofuranosidase protein1.760.701.44
SMb20946exoY, galactosyltransferase protein2.061.431.18
SMb20949exoV, putative pyruvyltransferase protein1.460.901.04
SMb20959exoO, glucosyltransferase protein0.701.121.37
SMb20960exoN, UDP-glucose pyrophosphorylase protein1.721.411.41
SMb20993Putative monooxygenase protein1.050.451.09
SMb21285Conserved hypothetical protein1.571.652.05
SMb21337Putative oxidoreductase1.431.480.65
SMb21432Putative iron ABC transporter periplasmic solute binding protein1.352.182.10
SMb21456Hypothetical protein1.881.330.60
SMb21464Putative transcriptional regulator GntR family protein1.070.101.42
SMb21487cyoA, putative cytochrome o ubiquinol oxidase chain II protein−0.512.882.94
SMb21488cyoB, putative cytochrome o ubiquinol oxidase chain I protein−0.292.762.55
SMb21489cyoC, putative cytochrome o ubiquinol oxidase chain III protein−0.092.642.08
SMb21690exoW, glucosyltransferase protein2.071.221.13
SMc00062Hypothetical protein1.050.881.63
SMc00086cycG, putative diheme cytochrome c type signal peptide protein1.90−0.011.52
SMc00136Putative oxidoreductase protein1.391.370.60
SMc00235trpD, probable anthranilate phosphoribosyltransferase protein1.44−0.061.74
SMc00301Conserved hypothetical protein0.401.021.31
SMc00338Conserved hypothetical protein1.781.230.82
SMc00371Conserved hypothetical protein1.091.110.27
SMc00401Conserved hypothetical protein1.721.702.99
SMc00402Putative iron-regulated protein A1.441.492.72
SMc00512Conserved hypothetical protein1.071.070.29
SMc00537Putative transport protein1.150.231.43
SMc00591Hypothetical/unknown signal peptide protein0.212.241.77
SMc00592Hypothetical transmembrane protein0.761.811.34
SMc00764Hypothetical or unknown transmembrane protein1.231.430.63
SMc00784Putative iron ABC transporter periplasmic binding protein1.422.862.94
SMc00819katA, catalase3.17−0.021.16
SMc00830Hypothetical protein−0.321.201.08
SMc00832glcD, probable glycolate oxidase subunit protein1.140.561.32
SMc00922Putative transporter transmembrane protein1.200.551.06
SMc01016Hypothetical protein1.091.750.71
SMc01022Putative cytochrome transmembrane protein2.161.001.28
SMc01095mexF1, probable multidrug efflux system transmembrane protein0.211.042.81
SMc01169ald, probable alanine dehydrogenase oxidoreductase protein1.231.480.56
SMc01266Conserved hypothetical protein1.481.480.98
SMc01267Conserved hypothetical protein0.911.311.13
SMc01471senC, putative cytochrome c oxidase assembly factor transmembrane protein−0.131.471.29
SMc01489Hypothetical protein2.091.301.04
SMc01512hmuT, putative hemin ABC transporter periplasmic binding protein1.331.323.00
SMc01513hmuS, Putative hemin ABC transporter ATPase2.393.044.62
SMc01514Conserved hypothetical protein2.203.565.01
SMc01516Conserved hypothetical protein1.341.772.54
SMc01517Conserved hypothetical protein0.531.182.58
SMc01658Putative transport protein1.360.983.11
SMc01659Putative iron ABC transporter periplasmic binding protein1.211.875.51
SMc01718Hypothetical transmembrane protein1.641.290.66
SMc01747hmuP, putative hemin transport protein2.301.523.68
SMc01765Hypothetical transmembrane protein0.901.431.04
SMc01788Hypothetical protein2.451.921.21
SMc01814Probable glutamate synthase small-chain protein0.152.151.17
SMc02047gcvT, probable aminomethyltransferase1.582.680.24
SMc02075Conserved hypothetical protein1.061.12−0.01
SMc02084exbD, putative polymer transport protein1.211.451.99
SMc02085exbB, putative polymer transport protein1.962.603.57
SMc02156Conserved hypothetical protein1.861.340.96
SMc02179Conserved hypothetical protein1.341.050.44
SMc02254qxtB, putative quinol oxidase subunit II1.092.661.08
SMc02255qxtA, putative quinol oxidase subunit I1.774.622.37
SMc02266Conserved hypothetical protein1.371.48−0.14
SMc02708Conserved hypothetical protein1.041.050.38
SMc02726shmR, hemin binding outer membrane receptor3.982.905.98
SMc02727Hypothetical protein1.980.462.23
SMc02887Hypothetical protein2.190.782.17
SMc03149Hypothetical protein0.341.381.58
SMc03787Hypothetical protein1.643.192.70
SMc03971mexF2, putative multidrug efflux system protein1.191.011.10
SMc04049Putative sulfite oxidase protein1.111.030.21
SMc04162Putative transcription regulator protein1.39−0.031.69
SMc04164Hypothetical protein1.401.560.92
SMc04206Putative hemolysin type calcium binding protein4.901.932.43
  • a Genes were considered differentially expressed when according to t-statistics the P value was <0.05 with an induction level of >twofold. Only genes differentially expressed during at least two sets of microarray experiments were considered.

  • b The expression ratios are expressed as log2 values.