TABLE 1.

Identification of carbaryl metabolites from the spent media of C4 culturesa

Identified metaboliteTLCUV-visible spectral properties (nm)bMS analysis {m/z (% relative intensity) [molecular ion]}
RfUV fluorescence
Carbaryl0.60Dark blue280, 312, 318201 (4) [M+], 144 (100), 127 (3), 115 (52), 89 (10), 77 (2)
1-Naphthol0.71Brown black298, 308, 323144 (100) [M+], 115 (77), 88 (10), 77 (2)
Salicylate0.78Sky blue306NDc
Gentisate0.18Blue-green338154 (37) [M+], 136 (100), 108 (10), 77 (1)
  • a Similar results were obtained with strains C5 and C6.

  • b Ethyl acetate was the solvent.

  • c ND, not detected by GC-MS.