TABLE 2.

PCR primers and annealing temperatures used in this study

PrimerGeneSequence (5′ to 3′)Tmaa (°C)
LBTTTTTTACACTGATGAATGTTCCGTT55
agfBF RTagfBTCCTGGTCTTCAGTAGCGTAA54.7
agfBR RTTATGATGGAAGCGGATAAGAA
agfDF RTagfDTCCTGGTCTTCAGTAGCGTAA59.1
agfDR RTTATGATGGAAGCGGATAAGAA
rplUF RTrplUCTGTTGAGTTCGCTGAAGTGC57.2
rplUR RTCAGCATTTGTGATAGCATTCGTCG
  • a Tma, annealing temperature used in PCR.