Plasmids used in this study

PlasmidDescriptionAntibiotic profileOriginReference or source
pTrc99AExpression vector under the control of the trc promoterAmprcol E11
pADSSynthetic ADS gene cloned into pTrc99A at NcoI-XmaIAmprcol E111
pMevTSingle operon under lac control coding for acetoacetyl-CoA thiolase, HMG-CoA synthase, and HMG-CoA reductaseCmrp1511
pMBISpBBR1MCS-2 with the genes for mevalonate kinase, phosphomevalonate kinase, diphosphomevalonate decarboxylase, IPP isomerase, and FPP synthase cloned as a single operonTcrBbr11
pNudFnudF cloned into pTrc99A at NcoI-XmaIcol E1This work
pYhfRyhfR cloned into pTrc99A at NcoI-XmaIcol E1This work
pC9bGenomic fragment from B. subtilis containing nudF cloned into pTrc99A at BamHIAmprcol E1This work