TABLE 1.

Primers and TaqMan probes used for this studya

GenePrimersTaqMan probeb
fri5′-CGGCGGAAGCCCATTC-3′FAM-5′-TATAAGGCGCTTCTTCTACGCTGGCATTTTCT-3′-QSY7
5′-CTAAGTCTTCCATTAATTGATCCATTGT-3′
ltrC5′-CCAGGCATTCTTGCAAAACTAA-3′FAM-5′-CCGTTCACACATTCCTTGA-3′-MGB-NFQ
5′-GATGGCGCCGACAATGTC-3′
oppA5′-GAAGATGCAAAATGGTCAAACG-3′FAM-5′-CCTGTAACTGCAAATGACTAT-3′-MGB-NFQ
5′-TGCACGACGCCATGAGTAAA-3′
  • a Primers and TaqMan probes for gap, rpoB, opuCA, and bsh have previously been described (33, 34).

  • b QSY7, a nonfluorescent quencher; MGB, minor groove binder; NFQ, 3′ nonfluorescent quencher; FAM, 6-carboxyfluorescein.