TABLE 2.

Sequence data for 29 clones of the enriched B. subtilis 6051 library

CloneGenome sectionaStrand orientationbComplete ORF(s)cStart bpd
a12/21+/+cypC16136
a218/21+/+opuCB, A53538
a36/21+/−yhfR98626
a46/21+/−yhfR98654
a56/21+/+yhfR96877
a613/21+/+nudF48012
a713/21+/+nudF48012
a810/21+/+none155264
a96/21+/+yhfR97154
a106/21+/−yhfR98713
b113/21+/+nudF48012
b26/21+/+yhfR96877
b36/21+/+yhfR96877
b46/21+/+yhfR97129
b66/21+/+yhfR96877
b76/21+/+yhfR96877
b86/21+/+yhfR96877
b96/21+/−yhfR98713
b1017/21+/+yutG, F, E, D102420
c113/21+/+nudF48012
c213/21+/+nudF47444
c318/21+/+chr, yvcK and L153828
c46/21+/+yhfR96877
c59/21+/−cheB, ylxH109331
c613/21+/+nudF48012
c713/21+/+nudF48012
c86/21+/−Fragments136685
c913/21+/−nudF49992
c1014/21+/+dnaJK11852
  • a Genome section numbers are for segments designated 1/21 to 21/21, corresponding to GenBank accession numbers Z99104 through Z99124, respectively; each segment represents 200 kb.

  • b The orientation of the cloned DNA with respect to the genome sequence is provided as +/+ or +/−. Sequences with +/+ orientation are cloned behind the trc promoter in the same direction as the reference genome sequence, whereas +/− oriented sequences are cloned in the reverse orientation.

  • c Annotated ORFs in the cloned region of the 5′ cloning junction. Note the dominance of yhfR- and nudF-containing clones.

  • d Base pair position of the 5′ cloning junction. The presence of multiple 5′ cloning sites indicates that yhfR- and nudF-containing clones arose independently from several genomic DNA library clones.