TABLE 2.

Genes identified by microarray analysis to be upregulated at 4°C in both log- and stationary-phase cellsa

Protein category and geneProtein function(s)bChange (n-fold) in cells grown toc:
Log phaseStationary phase
Cell membrane function
    lmo0540Similar to penicillin binding protein2.33.1
    lmo0581 (met)Putative S-adenosylmethionine-dependent methyltransferase4.72.2
    lmo1713Similar to cell-shape-determining proteins2.55.0
    lmo1864Similar to hemolysin III proteins, putative integral membrane protein4.18.3
    lmo2522Similar to hypothetical cell wall binding protein from B. subtilis10.64.5
Lipid and/or carbohydrate metabolism
    lmo0624Acetyltransferase, Gcn5-related N-acetyltransferase family3.62.1
    lmo0625Putative lipase/acylhydrolase2.73.1
    lmo1936 (gpsA)Similar to NAD(P)H-dependent glycerol-3-phosphate dehydrogenase2.24.2
    lmo2480Similar to acetyltransferase2.22.4
Motility
    lmo0677Similar to flagellar biosynthesis protein FliQ3.22.8
Signal transduction
    lmo0287Similar to two-component response regulator2.53.5
Transcription or translation
    lmo0866Similar to ATP-dependent RNA helicase2.43.0
    lmo1067Similar to GTP-binding elongation factor3.13.3
    lmo1364 (cspL)dSimilar to cold shock protein7.17.0
    lmo1722Similar to ATP-dependent RNA helicases2.43.9
Amino acid metabolism
    lmo0055 (purA)Highly similar to adenylosuccinate synthetase2.23.0
Other or hypothetical proteins
    lmo0047Unknown2.85.6
    lmo0189Highly similar to B. subtilis Veg protein12.25.2
    lmo0391Unknown3.84.1
    lmo0392Highly similar to B. subtilis YqfA protein3.12.8
    lmo0393Unknown3.73.8
    lmo0592Unknown3.42.4
    lmo0604Similar to B. subtilis YvlA protein2.83.5
    lmo0905Unknown2.53.0
    lmo0954Unknown2.85.0
    lmo1245Unknown4.74.6
    lmo1487Similar to unknown proteins2.33.0
    lmo1670Similar to conserved hypothetical proteins2.54.0
    lmo1937 (engA)GTP-binding protein2.65.4
    lmo2713Secreted protein with one GW repeat2.53.4
  • a Only the 30 genes that met the stringent criteria for being upregulated at 4°C in both log- and stationary-phase cells (i.e., a >2-fold change and an adjusted P value of <0.001) are listed here; all genes upregulated at 4°C in either log- or stationary-phase cells are listed in Tables S1 and S2 in the supplemental material.

  • b Protein functions are based on annotations provided by ListiList (http://genolist.pasteur.fr/ListiList/), TIGR (http://cmr.tigr.org/tigr-scripts/CMR/CmrHomePage.cgi), the NCBI (http://www.ncbi.nlm.nih.gov/), and the KEGG Sequence Similarity Database (http://www.genome.jp/kegg/ssdb/).

  • c Change (n-fold) indicates the transcript level ratio between L. monocytogenes 10403S cells grown at 4°C and those grown at 37°C (as determined by microarray analysis); positive values indicate that transcript levels are higher at 4°C than at 37°C (e.g., a value of 2.3 indicates a 2.3-fold-higher transcript level at 4°C than at 37°C).

  • d cspL has also been designated cspLA.