TABLE 1.

Sequences of primers and TaqMan probes used in this studya

GenePrimer sequencebTaqMan probe sequencec
lmo02875′-GCTCGTGTGAAAGCCAACTTG-3′FAM-5′-CCGTCACAGCCAAGT-3′-MGB NFQ
5′-TTCCTCGGCTGTGCTTGAG-3′
lmo08665′-GCGGTTTACGGTGGTAGTGATAT-3′FAM-5′-CGTCAAATCCGTTCACT-3′-MGB NFQ
5′-GGCGTACCAACTACGATTTGTG-3′
lmo1364 (cspL)5′-CCAAGGCGACGGATTCAA-3′FAM-5′-CAAGCAGTAACTTTCG-3′-MGB NFQ
5′-CGCGTTGGCCTTCTTCAA-3′
lmo17225′-CAAAGACGGTGCAGATGTACTTG-3′FAM-5′-AGTATCACCAACTGGAAC-3′-MGB NFQ
5′-GGCAGTGCATAAGCCACTGTT-3′
  • a The primers and TaqMan probes for rpoB and gap have previously been described (82, 88).

  • b The first sequence listed is that of the forward primer, and the second sequence listed is that of the reverse primer.

  • c FAM, 6-carboxyfluorescein; MGB, minor groove binder; NFQ, nonfluorescent quencher.