TABLE 4.

Detection of genotype-specific alleles of six SSR loci in 50 ng B. brongniartii-free bulk soil DNA spiked with mixtures of different quantities of three different genotypes of B. brongniartii

Spiked genotypeaTemplate quantityb (pg)MixturePresence (+) or absence (−) of alleles of locus:Retrieved genotypea
Bb1F4Bb2A3cBb2F8Bb4H9Bb5F4Bb8D6d
I2a++++++I
II20a++++++II
III20a++++++III
I0.2b++++UDe
II20b++++++II
III20b++++++III
I2c++++++I
II2c++++++II
III2c++++++III
I2d++++++I
II0.2d++UD
III0.2d+UD
I0.2e++UD
II0.2e+UD
III0.2e+UD
  • a According to Table 2.

  • b Based on genome size of B. bassiana (38), 2 pg of B. brongniartii genomic DNA corresponds to 45 genome copies.

  • c No discrimination of genotypes I and II possible (Table 2).

  • d No discrimination of the three genotypes possible (Table 2).

  • e UD, undefined.