TABLE 2.

Primers selected for detection of N. ceranae and N. apis in multiplex PCR

PrimerSequenceaPCR product size (bp)Specificity
218MITOC-FOR5′-CGGCGACGATGTGATATGAAAATATTAA-3′218-219bN. ceranae
218MITOC-REV5′-CCCGGTCATTCTCAAACAAAAAACCG-3′
321APIS-FOR5′-GGGGGCATGTCTTTGACGTACTATGTA-3′321N. apis
321APIS-REV5′-GGGGGGCGTTTAAAATGTGAAACAACTATG-3′
  • a CG tails added to primers are underlined.

  • b There is a 1-bp difference in the N. ceranae amplicon size depending on the sequences for N. ceranae available in GenBank (http://www.ncbi.nlm.nih.gov).