TABLE 5.

Monitoring of B. brongniartii in a BCA-treated plot (samples T1 to T5) and in a control plot (samples C1 to C5)

Soil sampleCultivation-dependent analysesiCultivation-independent analysesh
CFU g−1 soil (dry wt)No. of isolates genotypedGenotypeaDetection of SSR locus:Genotypea
Bb1F4Bb2A3Bb2F8Bb4H9Bb5F4Bb8D6
T114,383NAbNA+ (36)+ (40)+ (36)+ (32)+ (36)+ (32)I
T2229,2664I+ (36)+ (36)+ (32)+ (32)+ (32)+ (32)I
T39,4052I+ (36)+ (40)+ (36)+ (36)+ (36)+ (36)I
T4118,463NANA+ (32)+ (36)+ (32)+ (28)+ (28)+ (28)I
T5120,9048II+ (32)+ (36)+ (32)+ (32)+ (32)+ (32)II
C100NDc− (40)− (40)− (40)− (40)d− (40)d− (40)dND
C2724,4418III+ (28)+ (32)+ (28)+ (28)+ (28)+ (28)III
C32611III+ (40)− (40)− (40)+ (40)d+ (40)d+ (40)dUDCe
C400ND− (40)− (40)− (40)+f (40)d− (40)d+ (40)dUDg
C58161III− (40)− (40)− (40)− (40)d− (40)d− (40)dND
  • a According to Table 2.

  • b NA, not analyzed.

  • c ND, not detected.

  • d Analysis performed at Ca but repeated with 40 PCR cycles.

  • e UDC, undefined but consistent with genotype III.

  • f Allele of 215 bp, different from all three genotypes I, II, and III.

  • g UD, undefined.

  • h The presence (+) or absence (−) of SSR alleles and resulting genotypes was determined with cultivation-independent analyses of 50 ng bulk soil DNA of each sample. Numbers in parentheses are the numbers of cycles to which Ca values were set.

  • i Total CFU and genotypes of 24 selected isolates were determined with cultivation-dependent analyses.