TABLE 5.

Effect of fumarate on hydrogen utilization in rumen contents from conventional lambs C1 and C2 and meroxenic lambs with (lamb M5) and without (lambs M1 to M4) ruminal methanogens

Lamb age (mo)Incubation time (h)Hydrogen utilized (%) in incubationsa
Lambs M1 and M2Lambs M3 and M4Lamb M5Lambs C1 and C2
No fumarate18.7 mM fumarate37.4 mM fumarateNo fumarate18.7 mM fumarate37.4 mM fumarateNo fumarate18.7 mM fumarate37.4 mM fumarateNo fumarate18.7 mM fumarate37.4 mM fumarate
8838 ± 2 A43 ± 4 A60 ± 5 B30 ± 2 A27 ± 2 A40 ± 3 B72 ± 2 A82 ± 1.5 B82.7 ± 2 B76 ± 1 A80 ± 3 A82 ± 4 A
1248 ± 2 A59 ± 1 A,B69 ± 4 B32 ± 2 A30 ± 2.5 A44 ± 4 A92 ± 1.5 A96 ± 1.5 A96.6 ± 2 A95 ± 1 A96.7 ± 0.5 A97 ± 2 A
2493 ± 2 A95 ± 3 A100 A57 A61 ± 1.5 A58 ± 2.5 A100100100100100100
11830 ± 2 A45 ± 2 B53 ± 3 B30 ± 2NDbND42 ± 2 A51 ± 1 A63 ± 3 B55 ± 0.3 A55.8 ± 1 A57 ± 2 A
1245 ± 3 A60 ± 5 A,B72 ± 4 B50 ± 4 A72 ± 2 B87 ± 3 C74 ± 3 A75 ± 2 A76 ± 10 A73 ± 2 A77 ± 2.5 A81 ± 2 A
2468 ± 2 A78 ± 2 B82 ± 3 B70 ± 4 A77 ± 3 A90 ± 2 B100100100100100100
15836 ± 2 A47 ± 1 A,B57 ± 4 B29 ± 2 A31 ± 1 A34 ± 2 ANDNDND70 ± 2 A82 ± 2 B82 ± 1.5 B
1249 ± 2 A61 ± 3 B76 ± 3 C46 ± 4 A43 ± 2 A48 ± 1 ANDNDND96 ± 1 A97 ± 2 A97 ± 1 A
2495 ± 3 A99 ± 1 A100 A68 ± 3 A65 ± 2 A70 ± 3 ANDNDND100100100
18818 ± 2 A22 ± 3 AND16 ± 2 A18 ± 2 AND45 ± 4 A42 ± 2.5 AND46 ± 2.5 A45 ± 2ND
1225 ± 3 A27 ± 2 AND25 ± 2 A26 ± 2 AND58 ± 2 A58 ± 3 AND60 ± 3 A59 ± 2.5 AND
2460 ± 3 A66 ± 1 AND50 ± 2 A60 ± 2.5 BND99 ± 1 A98 ± 2 AND100100ND
  • a The values are means ± standard deviations for two samples. For each group of lambs, values followed by different letters in the same row differ significantly (P < 0.05).

  • b ND, not determined.