TABLE 3.

CT values for PCR amplification of six B. brongniartii SSR loci and corresponding CT-rel values

Value measuredTemplate DNAValuea for SSR locus:
Bb1F4Bb2A3Bb2F8Bb4H9Bb5F4Bb8D6
CTGenomic DNA from BCA strain31.93 ± 0.7534.57 ± 0.6531.07 ± 1.0728.83 ± 1.8630.97 ± 1.3129.30 ± 1.74
Spiked-soil DNA32.05 ± 0.8434.35 ± 1.0931.10 ± 0.7528.58 ± 0.2229.75 ± 0.5928.53 ± 0.68
CT-relGenomic DNA from BCA strain1.11 ± 0.041.20 ± 0.051.08 ± 0.061.00 ± 0.001.08 ± 0.071.02 ± 0.08
Spiked-soil DNA1.12 ± 0.021.20 ± 0.041.09 ± 0.021.00 ± 0.001.04 ± 0.020.99 ± 0.02
  • a CT-rel values were determined with reference to locus Bb4H9 derived either from 2 pg genomic DNA of the BCA strain (results are means of three replications) or from 50 ng bulk soil DNA spiked with 2 pg genomic DNA of the BCA strain (spiked-soil DNA) (results are means of four replications).