TABLE 3.

Sensitivity of the PCRs performed with the 218MITOC-FOR/218MITOC-REV and 321APIS-FOR/321APIS-REV primer pairs with spores diluted in water or uninfected honeybee maceratea

PrimersDiluentNo. positive/no. tested with the following no. of spores/100 μl (no. of spores/reaction mixture):No. positive/no. tested with:
3 × 106 (15 × 104)106 (5 × 104)105 (5 × 103)8 × 104 (4 × 103)6 × 104 (3 × 103)4 × 104 (2 × 103)2 × 104 (103)1.5 × 104 (750)104 (500)5 × 103 (250)2 × 103 (100)2 × 102 (10)20 (1)Uninfected honeybee macerateWater
218 MITOCWater6/66/66/66/66/66/65/65/63/60/60/60/60/60/60/6
Uninfected honeybee macerate6/66/66/66/66/66/63/61/61/60/60/60/60/60/60/6
321APISWater6/66/66/66/66/62/60/60/60/60/60/60/60/60/60/6
Uninfected honeybee macerate6/66/66/66/66/66/66/66/65/64/62/60/60/60/60/6
  • a All samples were analyzed by three PCRs performed on three different days, and the amplicon products of each PCR were separated by electrophoresis twice.