TABLE 1.

Detection specificity of six B. brongniartii SSR markers tested on a collection of 15 fungal reference strains, including ubiquitous soil fungal species, close relatives of B. brongniartii, and other entomopathogenic fungi

SpeciesOrderaFamilyaStrain collection accession no.bPresence or absence of SSR locusc:
Bb1F4Bb2A3Bb2F8Bb4H9Bb5F4Bb8D6
Beauveria brongniartiiHypocrealesClavicipitaceaeDSMZ 15205++++++
Beauveria bassianaHypocrealesClavicipitaceaeARSEF 5066+d
Hirsutella thompsoniiHypocrealesClavicipitaceaeARSEF 2800
Trichoderma harzianumHypocrealesHypocreaceaeDSMZ 63059
Tolypocladium cylindrosporumHypocrealesIncerta sedisARSEF 2777
Verticillium lecaniiHypocrealesIncerta sedisARSEF 1102
Metarhizium anisopliaeHypocrealesNectriaceaeARSEF 1066
Aspergillus parasiticusEurotialesTrichocomaceaeARSEF 5470
Paecilomyces fumosoroseusEurotialesTrichocomaceaeARSEF 1645
Penicillium chrysogenumEurotialesTrichocomaceaeACW 931
Conidiobolus coronatusEntomophthoralesAncylistaceaeIESR 04:427
Entomophthora culicisEntomophthoralesEntomorphtoraceaeARSEF 387
Erynia rhizosporaEntomophthoralesEntomorphtoraceaeARSEF 1440
Zoophthora radicansEntomophthoralesEntomorphtoraceaeARSEF 388
Mucor hiemalisMucoralesMucoraceaeACW 922
  • a According to the CABI Bioscience database.

  • b DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany; ARSEF, Agricultural Research Service Collection of Entomopathogenic Fungal Cultures, Ithaca, NY; ACW, Agroscope Changins-Wädenswil, Changins, Switzerland; IESR, Collection of Microorganisms, Institute of Environment and Sustainability Research, Staffordshire University, United Kingdom.

  • c Presence (+) or absence (−) of characteristic SSR marker signal.

  • d Amplicon length was 163 bp and different from that of B. brongniartii, which was 172 bp.