TABLE 1.

Detection specificity of six B. brongniartii SSR markers tested on a collection of 15 fungal reference strains, including ubiquitous soil fungal species, close relatives of B. brongniartii, and other entomopathogenic fungi

SpeciesOrderaFamilyaStrain collection accession no.bPresence or absence of SSR locusc:
Bb1F4Bb2A3Bb2F8Bb4H9Bb5F4Bb8D6
Beauveria brongniartii HypocrealesClavicipitaceae DSMZ 15205 ++++++
Beauveria bassiana HypocrealesClavicipitaceae ARSEF 5066 +d
Hirsutella thompsonii HypocrealesClavicipitaceae ARSEF 2800
Trichoderma harzianum HypocrealesHypocreaceae DSMZ 63059
Tolypocladium cylindrosporum HypocrealesIncerta sedis ARSEF 2777
Verticillium lecanii HypocrealesIncerta sedis ARSEF 1102
Metarhizium anisopliae HypocrealesNectriaceae ARSEF 1066
Aspergillus parasiticus EurotialesTrichocomaceae ARSEF 5470
Paecilomyces fumosoroseus EurotialesTrichocomaceae ARSEF 1645
Penicillium chrysogenum EurotialesTrichocomaceae ACW 931
Conidiobolus coronatus EntomophthoralesAncylistaceae IESR 04:427
Entomophthora culicis EntomophthoralesEntomorphtoraceae ARSEF 387
Erynia rhizospora EntomophthoralesEntomorphtoraceae ARSEF 1440
Zoophthora radicans EntomophthoralesEntomorphtoraceae ARSEF 388
Mucor hiemalis MucoralesMucoraceae ACW 922
  • a According to the CABI Bioscience database.

  • b DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany; ARSEF, Agricultural Research Service Collection of Entomopathogenic Fungal Cultures, Ithaca, NY; ACW, Agroscope Changins-Wädenswil, Changins, Switzerland; IESR, Collection of Microorganisms, Institute of Environment and Sustainability Research, Staffordshire University, United Kingdom.

  • c Presence (+) or absence (−) of characteristic SSR marker signal.

  • d Amplicon length was 163 bp and different from that of B. brongniartii, which was 172 bp.