TABLE 1.

Plasmids and bacterial strains used in this study

Plasmid or Rhizobium strainCharacteristics (amt of antibiotic used [μg/ml])aSource
pHC60Broad-host-range plasmid with gfp; Tcr (10)City University of New York (6)
Sinorhizobium meliloti 1021Smr (50)Institute of Plant Physiology, Shanghai, People's Republic of China
Azorhizobium caulinodans ORS 571Ampr (100), Cbr (100)Academy of Agriculture, Beijing, People's Republic of China
Sinorhizobium meliloti USDA 1002Kmr (50)Chinese University of Agriculture, Beijing, People's Republic of China
Rhizobium leguminosarum USDA 2370Smr (50)Chinese University of Agriculture, Beijing, People's Republic of China
Mesorhizobium huakui 93Smr (10)Nanjing University of Agriculture, Nanjing, People's Republic of China
Sinorhizobium meliloti 1021 (gfp)Smr (50), Tcr (10)City University of New York, New York, N.Y.
Azorhizobium caulinodans ORS 571 (gfp)Ampr (100), Tcr (10)This study
Sinorhizobium meliloti USDA 1002 (gfp)Kmr (50), Tcr (10)This study
Rhizobium leguminosarum USDA 2370 (gfp)Smr (50), Tcr (10)This study
Mesorhizobium huakui 93 (gfp)Smr (10), Tcr (10)This study
  • a Tcr, tetracycline resistance; Smr, streptomycin resistance; Cbr, carbenicillin resistance; Kmr, kanamycin resistance; Ampr, ampicillin resistance; Tcr, tetracycline resistance.