TABLE 1.

Environmental variables, total abundance of DAPI-positive cells, and bacterial community compositions at coastal sampling stations

Location (date, day/mo/yr)Station (depth, m)Water temp (°C)Chlorophyll aa (μg liter−1)Total abundance of DAPI-positive cells (106 cells ml−1)% of total bacterial abundanceb (avg ± SE, n = 10)
EubAlphaBetaGammaCFΣ
Otsuchi Bay (22/05/01)A (0)12.91.771.457 ± 133 ± 14 ± 18 ± 113 ± 158
A (20)9.61.651.657 ± 161 ± 16 ± 110 ± 118 ± 195
B (0)12.92.421.854 ± 217 ± 18 ± 1<16 ± 133
B (40)8.73.091.543 ± 217 ± 13 ± 17 ± 14 ± 131
C (0)10.41.181.551 ± 233 ± 1<17 ± 117 ± 157
C (65)8.51.681.122 ± 133 ± 1<1<18 ± 141
Otsuchi Bay (24/05/02)A (0)13.41.921.554 ± 139 ± 1<16 ± 013 ± 158
B (0)13.42.561.551 ± 246 ± 1<18 ± 112 ± 166
C (0)11.71.851.223 ± 222 ± 3<1<1<126
Sagami Bay (17/07/02)1 (0)21.93.431.549 ± 136 ± 1<16 ± 18 ± 150
1 (100)16.00.090.327 ± 235 ± 2<18 ± 17 ± 150
Off Kuroshio (18/07/02)4 (0)27.60.180.717 ± 127 ± 1<1<1<127
4 (100)22.40.120.314 ± 123 ± 1<1<15 ± 128
Total40 ± 532 ± 43 ± 25 ± 19 ± 249 ± 6
  • a Fluorometric measurement after the extraction of pigments with N,N-dimethylformamide (H. Ogawa and H. Fukuda, personal communication).

  • b Bacteria were classified by using rRNA binding oligonucleotide probes specific for eubacteria (Eub), α-proteobacteria (Alpha), β-proteobacteria (Beta), γ-proteobacteria (Gamma), and the Cytophaga-Flavobacter cluster (CF). Σ, sum of four phylogenetic bacterial groups.