TABLE 1.

PCR primer sequences, targets, annealing temperatures, and amplicon lengths

PrimerClass targetedPrimer sequence (5′→3′)Primer annealing temp (°C)cAmplicon size (bp)Reference
tetAC-150faTet A, CGCT RTA TGC GTT GRT GCA AT58567This study
tetAC-716raTet A, CTCC TCG CCG AAA ATG ACC
tetG-247fbTet GGTC GAT TAC ACG ATT ATG GC57432This study
tetG-678rbTet GCAC TTG GCC GAT CAG TTG A
Ribo2-FWTet M, O, P, Q, S, T, WGGM CAY RTG GAT TTY WTI GC521,3155
Ribo2-RVTet M, O, P, Q, S, T, WTCI GMI GGI GTR CTI RCI GGR C
  • a Numbered according to the tet(A) gene in plasmid pRP1 in E. coli (GenBank accession no. X00006).

  • b Numbered based on the tet(G) gene in plasmid pSTG1 in a Pseudomonas sp. (GenBank accession no. AF133139).

  • c For touchdown PCR. See the text for details.