TABLE 1.

E. coli K12 strains and plasmids

Strain or plasmidGenotype and/or properties (reference); source
Strains
    PC2leuB6 thyA47 dnaC2 dnaT12 deoC3 rpsL153 λ (5); laboratory stock
    SS1020λ rph-1 zjj202::Tn10 dnaC2; Steven Sandler
    HME5F λ IN (rrnD-rrnE) ΔlacU169 λcI857 Δ(cro-bioA) (32); Donald Court
    MC1061araD139 Δ(ara, leu)7697 ΔlacX74 galU galK rpsL hsdR2 (rK− mK+) mcrB1; laboratory stock
    KH1061araD139 Δ(ara, leu)7697 ΔlacX74 galU galK rpsL hsdR2 (rK− mK+) mcrB1 ΔdnaC::cat; this work
    MF1061araD139 Δ(ara, leu)7697 ΔlacX74 galU galK rpsL hsdR2 (rK− mK+) mcrB1 ΔdnaC::cat recA::Kanr; Magdalena M. Felczak of this laboratory by P1 transduction from BW26355 (relevant genotype, ΔrecA635::Kanr) (8) into MC1061, followed by selection for kanamycin resistance; the transductant and BW26355 were confirmed to be UV sensitive
Plasmids
    pACYC184cat, Tetr (6)
    pACYC184dnaCTetr, dnaC (this work)
    pAM34Ampr, IPTG-dependent maintenance (32); Kaymeuang Cam
    pAM34dnaCL1-2Ampr, IPTG-dependent maintenance, dnaC (this work)
    pAM34dnaCL1-4Ampr, IPTG-dependent maintenance, dnaC (this work)
    pINCSSDKanr, pBR322 derivative, dnaC under araBAD promoter control and downstream from the ribosome binding site for gene 10 of T7 phage (1)
    pdnaC113Ampr, pBR322 derivative, dnaC under araBAD promoter control (20)
    pdnaC97 (Pro108Ser)Kanr, pINCSSD derivative, missense mutation encoding Pro108Ser substitution (20)
    pdnaC116 (Leu44Pro)Kanr, pINCSSD derivative, missense mutation encoding Leu44Pro substitution (20)
    pdnaC250 (Leu11Gln)Kanr, pINCSSD derivative, missense mutation encoding Leu11Gln substitution (20)
    pHK-dnaCAmpr, pET11a derivative (Novagen), dnaC joined at the 5′ end to sequence encoding protein kinase A phosphorylation site and six histidine residues (9); Mike O'Donnell
    pHK-dnaCK112RAmpr, derivative of pHK-dnaC encoding a Lys112Arg substitution (9); Mike O'Donnell