TABLE 2.

Primers and annealing temperatures used for gene-specific PCR amplification and sequencing of L. lactis isolates

GeneForward primeraReverse primeraAnnealing temp (°C)
rpoA5′-ATGATYGARTTTGAAAAACC-3′5′-ACHGTRTTRATDCCDGCRCG-3′46
atpA5′-TAYRTYGGKGAYGGDATYGC-3′5′-CCRCGRTTHARYTTHGCYTG-3′50
bcaT5′-TTTKSHRTGCCDGTWGG-3′5′-GGWCCHACTTCYGTYTC-3′46
pepN5′-ATKTCTTAYGCWGAYRTYGT-3′5′-TTKCTTCAAGSMAWGSCC-3′50
pepX5′-TTTGGGTTGAAAGTCCAGT-3′5′-CCAAGAAGAAATTCCAGC-3′46
pheS5′-CAYCCNGCHCGYGAYATGC-3′5′-CCWARVCCRAARGCAAARCC-3′50
SSU rRNA5′-GCGGCGTGCCTAATACATGC-3′5′-ATCTACGCATTTCACCGCTAC-3′50
  • a Y, R, H, D, K, Y, W, M, and N are degenerate nucleotides according to the degenerate nucleotide alphabet: R for A or G; M for A or C; Y for C or T; K for G or T; W for A or T; N for A, G, C or T; S for G or C; H for A, C or T; and D for A or G or T.