TABLE 3.

Results of anaerobic fermentation of E. coli W3110 and its various mutant strains

StrainFermentation time (h)OD600Concn of substrate or product (mM)aSuccinic acid ratiocFold changed
GlucosebSuccinic acidLactic acidFormic acidAcetic acidEthanol
W3110241.79 ± 0.115.07 ± 0.452.43 ± 0.0310.62 ± 2.4288.03 ± 0.4240.10 ± 0.205.77 ± 0.060.0171.00
Strains constructed based on metabolic pathway comparison
W3110G241.47 ± 0.015.66 ± 1.772.16 ± 0.0813.71 ± 3.4688.57 ± 2.9740.69 ± 0.975.98 ± 0.090.0140.86
W3110GF241.46 ± 0.044.28 ± 1.422.83 ± 0.0714.13 ± 1.3286.25 ± 2.0240.10 ± 0.585.92 ± 0.240.0191.15
W3110GFO241.49 ± 0.074.68 ± 0.352.67 ± 0.3312.97 ± 0.0688.39 ± 0.8140.89 ± 0.186.00 ± 0.080.0181.07
W3110GFH241.35 ± 0.014.70 ± 0.392.51 ± 0.0215.18 ± 1.4985.86 ± 0.1038.88 ± 0.165.84 ± 0.060.0171.02
W3110GFOH241.28 ± 0.114.82 ± 0.482.58 ± 0.0317.06 ± 0.4590.40 ± 2.5540.40 ± 0.496.20 ± 0.060.0161.00
W3110GFOHE241.27 ± 0.054.25 ± 0.272.49 ± 0.1813.31 ± 0.7885.98 ± 0.3838.66 ± 0.025.84 ± 0.010.0171.03
Strains constructed based on in silico simulation resuts
W3110GFA240.73 ± 0.0620.57 ± 3.028.16 ± 0.015.47 ± 0.4927.47 ± 2.9416.48 ± 1.551.88 ± 0.240.1378.29
W3110GFA800.99 ± 0.040.41 ± 0.0517.35 ± 0.0310.67 ± 0.5350.36 ± 3.0030.25 ± 1.474.14 ± 0.420.1509.23
W3110GFAP800.682 ± 0.1612.2 ± 0.048.45 ± 0.0521.4 ± 0.88NDe4.1 ± 0.045.5 ± 0.330.21412.60
W3110GFAPL800.05 ± 0.0241.6 ± 0.80.39 ± 0.01NDNDND1.6 ± 0.070.1911.53
  • a Anaerobic vial fermentation on 50 mM initial glucose for 24 or 80 h.

  • b Residual glucose concentration.

  • c Calculated as succinic acid/(succinic acid + lactic acid + formic acid + acetic acid + ethanol).

  • d Calculated as succinic acid ratio/succinic acid ratio in the wild-type strain (0.017).

  • e Not detected.