TABLE 2.

Oligonucleotides used for gene replacement experiments

TemplatePCR no.PrimerSequence (5′ → 3′)
SpR1PTSG1TGC CCG CCG TTG TAT CGC ATG TTA TGG CAG GGG GAT CGA TCC TCT AGA
PTSG2TGC AGC AAC CAG AGC CGG TGC CAT TTC GCT GGG CCG ACA GGC TTT
2PTSG3TGG GCG TCG GTT CCG CGA ATT TCA GCT GGC TGC CCG CCG TTG TAT CGC
PTSG4GAG GTT AGT AAT GTT TTC TTT ACC ACC AAA TGC AGC AAC CAG AGC CGG
TcR1PYKF1TGG ACG CTG GCA TGA ACG TTA TGC GTC TGA GGG TAG ATT TCA GTG CAA
PYKF2CGC CTT TGC TCA GTA CCA ACT GAT GAG CCG GGG TTC CAT TCA GGT CGA
2PYKF3CCG AAT CTG AAG AGA TGT TAG CTA AAA TGC TGG ACG CTG GCA TGA ACG
PYKF4AAG TGA TCT CTT TAA CAA GCT GCG GCA CAA CGC CTT TGC TCA GTA CCA
CmR1MQO1GGC ATA CCA TGC CGG ATG TGG CGT ATC ATT GGG GTT TAA GGG CAC CAA
MQO2GAA CTA CGG CGA GAT CAC CCG CCA GTT AAT GCC CCG GGC TTT GCG CCG
2MQO3TGG CGC GTC TTA TCA GCA TAC GCC ACA TCC GGC ATA CCA TGC CGG ATG
MQO4AGC CAC GCG TAC GGA AAT TGG TAC CGA TGT GAA CTA CGG CGA GAT CAC
KmR1SDH1CAG TCA GAG AAT TTG ATG CAG TTG TGA TTG ATC GGG GGG GGG GGA AAG
SDH2ATC GGC TCT TTC ACC GGA TCG ACG TGA GCG ATC CCA ATT CTG ATT AGA
2SDH3GTT GTG GTG TGG GGT GTG TGA TGA AAT TGC CAG TCA GAG AAT TTG ATG
SDH4ATC ATG TAG TGA CAG GTT GGG ATA ACC GGA ATC GGC TCT TTC ACC GGA
PmR1ACEBA1GCA CCT TGT GAT GGT GAA CGC ACC GAA GAA CGA GCT CGG TAC CCG GGC
ACEBA2CTT TCG CCT GTT GCA GCG CCT GAC CGC CAG CAA TAG ACC AGT TGC AAT
2ACEBA3GAC GCG CCG ATT ACT GCC GAT CAG CTG CTG GCA CCT TGT GAT GGT GAA
ACEBA4ATC CCG ACA GAT AGA CTG CTT CAA TAC CCG CTT TCG CCT GTT GCA GCG
KmR1PYKA1CAC CTG GTT GTT TCA GTC AAC GGA GTA TTA CAT CGG GGG GGG GGG AAA G
PYKA2GTG GCG TTT TCG CCG CAT CCG GCA ACG TAC ATC CCA ATT CTG ATT AG
2PYKA3TTA TTT CAT TCG GAT TTC ATG TTC AAG CAA CAC CTG GTT GTT TCA GTC
PYKA4GTT GAA CTA TCA TTG AAC TGT AGG CCG GAT GTG GCG TTT TCG CCG CAT C
PmR1LDHA1CGG TGT GGC GAT GCT GCG CAT TCT GAA AGG CAA TAG ACC AGT TGC AAT
LDHA2GTC AGA GCT TCT GCT GTC AGG AAT GCC TGG CGA GCT CGG TAC CCG GGC
2LDHA3AAC GGC AGG CGT TAT CGG TAC CGG TAA AAT CGG TGT GGC GAT GCT GCG
LDHA4AAG TTT TGC AGC GTA GTC TGA GAA ATA CTG GTC AGA GCT TCT GCT GTC
CmR1PFLB1GGG GTG TTA CCC GTT TTC TTA CCA TAC ACA GGG GTT TAA GGG CAC CAA
PFLB2AGT GGA CTT ACT TCG GCT ACC TGG CTG CTG GGG CTT TGC GCC GAA TAA
2PFLB3CCG AAC GGC GCG CCA GCA CGA CGA CCG TCT GGG GTG TTA CCC GTT TTC
PFLB4GTC CGG CTA CCA ACG CTC AGG AAG CTA TCC AGT GGA CTT ACT TCG GCT