TABLE 3.

Predicted BT NRPS module substrate specificities and refinement of the BT1583 peptide structure

ModuleAmino acid at PheA residuea:Predicted substrate specificityPartial BT1583 sequenceRefined BT1583 sequence
235236239278299301322330331517
1DFWNIGMVHKThr/Dht(CH3)2-Bmt?b(CH3)2-Bmt?
2DGFLLGGVFKIle/LeuLeu/IleLeuc
3DSGPSGAVDKOrnbOrn
4DGFFLGVVYKIlebLeu/IleIle
5DGFFVGGVFKIle/Leu/ValValbVal
6DGFFVGGVFKIle/Leu/ValValbVal
7DAGPSGAVDKLysbLys
8DGFFVGGVFKIle/Leu/ValValbVal
9DAWFLGNVVKLeubLeu/IleLeu
10DAGPSGAVGKLysbLys
11DAAAVVGVAKPhe/Trp/TyrTyrbTyr
12DAWFLGNVWKLeubLeu/IleLeu
13DGFFAGGVFKIle/Leu/ValVal-CH2OHbVal-CH2OH
  • a The residues were numbered according to the corresponding residues of PheA (4).

  • b The information was used for BT1583 peptide sequence refinement.

  • c The Leu at this position was deduced from the fact that the only Ile had been assigned to position 4.