TABLE 3.

Diversity among 16S rRNA gene clonesa

CloneGenBank accession no.Closest relative in GenBank; accession no.Similarity (%)CladeGroup
Control
    BG27-10AY904490Uncult. Alphaproteobacteria clone PI_RT68; AY58044599.4RoseobacterAlpha
    BG27-14AY904494Uncult. Alphaproteobacteria clone CD2AC12; AY03857199.8RoseobacterAlpha
    BG27-16AY904495Isolate SRF3; AJ00256599.8RoseobacterAlpha
    BG27-4AY904484Uncult. Alphaproteobacteria clone MB-C2-128; AY09348198.8SAR11Alpha
    BG27-5AY904485Uncult. Alphaproteobacteria clone MB11B07; AY03329997.1SAR11Alpha
    BG27-6AY904486Uncult. Alphaproteobacteria clone EBAC40E09; AF26822098.9SAR11Alpha
    BG27-8AY904488Uncult. Alphaproteobacteria clone RAI-14; AY49941699.6SAR11Alpha
    BG27-12AY904492Uncult. Alphaproteobacteria clone PI_RT324; AY58047499.8SAR11Alpha
    BG27-13AY904493Uncult. Alphaproteobacteria clone PI_4g12g; AY58048399.3SAR11Alpha
    BG27-19AY904498Uncult. Alphaproteobacteria clone PLY43; U1315999.5SAR11Alpha
    BG27-20AY904499Uncult. Alphaproteobacteria clone F2CB7; AY697876100SAR11Alpha
    BG27-1AY904481Uncult. Deltaproteobacteria clone JL-WNPG-T23; AY66412097.9NDDelta
    BG27-7AY904487Uncult. Gammaproteobacteria clone BTM43; AY19315791.8NDGamma
    BG27-17AY904496Uncult. Gammaproteobacteria clone HRV1; Z8857998.5OM60Gamma
    BG27-2AY904482Isolate, Synechococcus sp. strain WH 8109; AY17283699.6SynechococcusCyanobacteria
    BG27-9AY904489Identical to BG27-2, Synechococcus sp. strain WH 810999.6SynechococcusCyanobacteria
    BG27-18AY904497Identical to BG27-2, Synechococcus sp. strain WH 810999.6SynechococcusCyanobacteria
    BG27-3AY904483Isolate, Synechococcus sp. strain WH 8109; AY17283699.2SynechococcusCyanobacteria
    BG27-11AY904491Uncult. actinobacteria clone MB11A03; AY033296100SAR432Actinobacteria
Enriched
    BG29-3AY904502Isolate K2-53B; AY34541396.8RoseobacterAlpha
    BG29-4AY904503Isolate RED15; AY13612498.7RoseobacterAlpha
    BG29-7AY904506Uncult. Alphaproteobacteria clone 128-19L; AF47391597.9RoseobacterAlpha
    BG29-9AY904508Uncult. Alphaproteobacteria clone CD4C7; AY03853197.1RoseobacterAlpha
    BG29-10AY904509Isolate HRV4# HpaAS2; Z8858299.6RoseobacterAlpha
    BG29-15AY904513Uncult. Alphaproteobacteria clone PI_RT68; AY58044599.6RoseobacterAlpha
    BG29-13AY904512Uncult. Alphaproteobacteria clone MB11B03; AY03329899.1SAR116Alpha
    BG29-18AY904516Uncult. Roseospirillum sp. clone 1B9; AJ62798790.6NDAlpha
    BG29-5AY904504Uncult. Gammaproteobacteria clone PI_4r3b; AY58081596.5NDUnknown
    BG29-16AY904514Uncult. Gammaproteobacteria clone JH10_C68; AY56882093.7OM60Gamma
    BG29-17AY904515Uncult. Gammaproteobacteria clone CRO-FL8; AF14158696.4SAR92Gamma
    BG29-20AY904518Isolate HTCC2080; AY38633993.4OM60Gamma
    BG29-1AY904500Uncult. Gammaproteobacteria clone HRV33; Z8858999.2SAR86Gamma
    BG29-19AY904517Uncult. Gammaproteobacteria clone HRV33; Z8858999.1SAR86Gamma
    BG29-6AY904505Uncult. Proteobacteria clone CD5F2; AY03841589.3NDUnknown
    BG29-2AY904501Uncult. Synechococcus sp. clone A715015; AY12537199.4SynechococcusCyanobacteria
    BG29-11AY904510Isolate, Synechococcus sp. strain WH 8109; AY17283699.5SynechococcusCyanobacteria
    BG29-8AY904507Uncult. Planctomycetes clone K2-30-19; AY34441288.2NDPlanctomycetes
    BG29-12AY904511Uncult. Planctomycetes clone PI_4e8e; AY58043393.2NDPlanctomycetes
    BG29-14AY573531Psychroserpens burtonensis ACAM188; U6291394.4FlavobacteriaBacteroidetes
  • a Clone libraries from the control microcosm C1 and the enriched microcosm NUT1 were constructed from community DNA samples collected on day 7. Alpha, Alphaproteobacteria; Gamma, Gammaproteobacteria; Delta, Deltaproteobacteria; ND, not determined; Uncult., uncultured.