TABLE 2.

Rate coefficients and enantioselectivity of transformation derived from the incubation experiments with E. coli BL21 expressing GST-fused LinA1 and LinA2 and with whole cells of S. paucimobilis B90Aa

Rate coefficient or ESIncubation with:
E. coli BL21 (pLINEA1)E. coli BL21 (pLINEA2)S. paucimobilis B90A
Transformation of     α-HCH
    k+ (h−1)0.45 ± 0.030.040 ± 0.0061.1 ± 0.2
    k (h−1)0.060 ± 0.0100.47 ± 0.061.2 ± 0.2
    ES0.76 ± 0.08−0.84 ± 0.15−0.09 ± 0.13
Transformation of     β-PCCH
    kβ-PCCH-1 (h−1)0.078 ± 0.0091.4 ± 0.94.5 ± 2.8
    kβ-PCCH-2 (h−1)0.24 ± 0.080.089 ± 0.0083.2 ± 1.5
  • a The rate constants were obtained by fitting the data to the reaction scheme shown in Fig. 5.