TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
X. nematophila strains
    HGB800X. nematophila ATCC 19061, wild typeAmerican Type Culture Collection
    HGB1227HGB800 (Tn7)This study
    HGB1228HGB800 (Tn7/cpxRA)This study
    HGB1230HGB800 ΔcpxR1This study
    HGB1231HGB800 ΔcpxR1 (Tn7)This study
    HGB1232HGB800 ΔcpxR1 (Tn7/cpxRA)This study
    HGB1236HGB800 (Tn7/cpxRp-lacZ)This study
    HGB1237HGB800 ΔcpxR1 (Tn7/cpxRp-lacZ)This study
    HGB1117HGB800 (Tn7/nilA-lacZ)11
    HGB1121HGB800 (Tn7/nilAB-lacZ)11
    HGB1101HGB800 (Tn7/nilC-lacZ)10
    HGB1239HGB800 ΔcpxR1 (Tn7/nilA-lacZ)This study
    HGB1240HGB800 ΔcpxR1 (Tn7/nilAB-lacZ)This study
    HGB1241HGB800 ΔcpxR1 (Tn7/nilC-lacZ)This study
E. coli strains
    S17-1(λpir)Donor strain for conjugations72
    SM10Donor strain for conjugations of Strr plasmids72
Plasmids
    pCR2.1-TOPOCloning vector, Ampr KanrInvitrogen (Carlsbad, CA)
    pKR100oriR6K, mobRP4 suicide vector, CmrK. Visick, Loyola University
    pKJN102Source of aad (Strr) geneK. Cowles, University of Wisconsin—Madison
    pKRcpxRuppKR100 plus 1,058-bp cpxR 5′ flanking regionThis study
    pKRcpxRupStrpKRcpxRup plus aad (Strr) geneThis study
    pKRcpxRStrpKRcpxRupStr plus 1,288-bp cpxR 3′ flanking regionThis study
    pEVS107Tn7 transposon delivery vector, oriR6K, mobRP4, Ermr Kanr46
    pEVSCmDerivative of pEVS107; Tn7 transposon vector, Ermr CmrE. Martens, University of Wisconsin—Madison
    pEVSCmKanDerivative of pEVS107; Tn7 transposon vector, Cmr KanrE. Martens, University of Wisconsin—Madison
    pUX-BF13Triparental mating helper plasmid expressing Tn7 transposase, Ampr4
    pTn7/nilA-lacZpEVS107 plus nilA 5′ flanking region fused to lacZ, Ermr Kanr11
    pTn7/nilAB-lacZpEVS107 plus nilB 5′ flanking region fused to lacZ, Ermr Kanr11
    pTn7/nilC-lacZpEVS107 plus nilC 5′ flanking region fused to lacZ, Ermr Kanr10
    pTOPOcpxRAcomppCR2.1-TOPO plus cpxRA, including 152 bp of 5′ regionThis study
    pTn7cpxRAcomppEVSCm plus cpxRA and 152 bp of cpxRA 5′ regionThis study
    pKV124Source of promoterless lacZ for mini-Tn7 fusions, Cmr76
    pTOPOcpxRppCR2.1-TOPO plus 152 bp of cpxR 5′ flanking regionThis study
    pKVTOPOcpxRppKV124 fused to pTOPOcpxRp, placing ′lacZ downstream of cpxRpThis study
    pTn7/cpxRp-lacZpEVSCmKan plus cpxRp-lacZ fragmentThis study