TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaSource or reference
E. coli strains
    W3110Coli Genetic Stock Center strain (CGSC) no. 4474CGSC
    W3110GptsG::SprThis study
    W3110GFptsG::Spr pykF::TcrThis study
    W3110GFOptsG::Spr pykF::Tcr mqo::CmrThis study
    W3110GFHptsG::Spr pykF::Tcr sdh::KmrThis study
    W3110GFHOptsG::Spr pykF::Tcr sdh::Kmr mqo::CmrThis study
    W3110GFHOEptsG::Spr pykF::Tcr sdh::Kmr mqo::Cmr aceBA::PmrThis study
    W3110GFAptsG::Spr pykF::Tcr pykA::KmrThis study
    W3110GFAPptsG::Spr pykF::Tcr pykA::Kmr pflB::CmrThis study
    W3110GFAPLptsG::Spr pykF::Tcr pykA::Kmr pflB::Cmr ldhA::PmrThis study
Plasmids
    pKD46Red recombinase expression plasmid, temperature-sensitive ori; Apr5
    pACYC184Cmr TcrNew England Biolabsb
    pIC156Spr26
    pUC19-phleoApr Pmr7
    pUC4KApr KmrPharmaciac
  • a Abbreviations: Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin; Pm, phleomycin; Sp, spectinomycin; Tc, tetracycline.

  • b New England Biolabs, Inc., Beverly, Mass.

  • c Pharmacia Biotech, Uppsala, Sweden.