TABLE 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionSource or reference
Escherichia coli
    CC118 λpirΔ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA λpir lysogen23
    S17-1 λpirTp Sm thi pro recA HsdR HsdM+; RP4-2-Tc::Mu::Km Tn7 λpir23
    XL1-Blue MRF′(mcrA)183 (mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proABlacIqZ.M15 Tn5(Kan)]Stratagene, La Jolla, CA
Edwardsiella ictaluri
    93-146Wild-type E. ictaluri isolated in 1993 from moribund channel catfish in a natural outbreak of ESC on a commercial farmLSU aquatic animal diagnostic laboratory
Plasmids
    pBluescript SK-Cloning vectorStratagene, La Jolla, CA
    pUT-miniTn5Km2Delivery plasmid carrying mini-Tn5Km23
    pUT-miniTn5Km-MCSpUT-miniTn5Km2 with multiple cloning site containing EcoRV, XbaI, and ApaI restriction enzyme sitesThis study
    pUT-Km-STMpUT-miniTn5Km-MCS carrying a specific tag in the MCSThis study