TABLE 1.

Kinetic constants of E. coli-expressed intact G2 EPSPS and reconstituted G2 EPSPSa

EnzymeMean sp act (U/mg) ± SDKm (PEP) (μM) ± SDKi (glyphosate) (μM) ± SDKi/Km (PEP) ± SDVmax (U/mg) ± SD
Intact G2 EPSPS7.12 ± 0.1395.20 ± 5.7349.5 ± 3.170.527.24 ± 0.16
Reconstituted G2-EPSPS4.48 ± 0.7996.80 ± 11.0135.0 ± 5.780.364.761 ± 0.92
EPSPSN295INNDNDNDND
ICEPSPSC296NDNDNDND
  • a The results presented are averages of two sets of experiments performed in triplicate. —, the specific enzyme activity is less than 0.1% of that of intact enzyme; ND, not determined.