TABLE 1.

bla CMY-2 containing S. enterica and E. coli isolates

IsolateSerotypeaPFGE typeResistance phenotypebPlasmid typeblaCMY-2 fragment size (kb)dConjugativeeYr
Source strainTransformant
S8126DublinD1AMP CHL GEN KAN SXT TET SSS STR CAZSame as donorc320N2003
S8133DublinD1AMP CHL GEN KAN SXT TET SSS STR CAZSame as donor320N2002
S8244DublinD2AMP CHL TET SSS STR CAZSame as donor720N2003
S8247DublinD1AMP CHL GEN KAN TET SSS STR CAZAMP CHL KAN TET SSS CAZ320Yf2003
S8253DublinD2AMP CHL TET SSS STR CAZSame as donor720N2003
S8274DublinD1AMP CHL GEN KAN TET SSS STR CAZSame as donor320N2003
S8282DublinD3AMP CHL GEN KAN TET SSS STR CAZSame as donor320N2002
S7651MuensterM1AMP CHL GEN KAN SXT TET SSS STR CAZSame as donor23.2N2002
S7140NewportN3AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7276NewportN2AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7333NewportN2AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7465NewportN2AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7497NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7656NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7658NewportN8AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7661NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7677NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7748NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7885NewportN7AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7889NewportN2AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7891NewportN11AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7893NewportN2AMP TET SSS STR CAZSame as donor333.2N2002
S7894NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7906NewportN10AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7909NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7918NewportN13AMP TET SSS STR CAZSame as donor313.2N2002
S7926NewportN2AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7935NewportN2AMP CHL TET SSS STR CAZSame as donor183.2N2002
S7936NewportN12AMP CHL GEN SXT TET SSS STR CAZAMP CHL TET SSS CAZ183.2N2002
S8118NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2002
S8124NewportN3AMP CHL TET SSS STR CAZSame as donor323.2N2002
S8129NewportN3AMP CHL TET SSS STR CAZSame as donor1320N2003
S8132NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2003
S8151NewportN9AMP CHL TET SSS STR CAZSame as donor1320N2003
S8242NewportN4AMP CHL TET SSS STR CAZSame as donor183.2N2003
S8245NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2003
S8257NewportN5AMP TET SSS STR CAZSame as donor313.2N2003
S8258NewportN1AMP CHL TET SSS STR CAZSame as donor183.2N2003
S8277NewportN4AMP CHL TET SSS STR CAZSame as donor183.2N2003
S8280NewportN2AMP CHL TET SSS STR CAZSame as donor183.2N2003
S8281NewportN2AMP CHL TET SSS STR CAZSame as donor183.2N2003
S8912NewportN6AMP TET SSS STR CAZSame as donor303.2N2003
S8922NewportN11AMP TET SSS STR CAZSame as donor323.2N2003
S7655TyphimuriumT2AMP CHL KAN SXT TET SSS STR CAZSame as donor1420N2002
S7907TyphimuriumT4AMP CHL KAN SXT TET SSS STR CAZSame as donor1320N2002
S8238TyphimuriumT1AMP CHL TET SSS STR CAZSame as donor293.2N2003
S8240TyphimuriumT5AMP CHL KAN SXT TET SSS STR CAZAMP CHL SXT TET SSS CAZ1120N2003
S8914TyphimuriumT3AMP CHL KAN TET SSS STR CAZSame as donor1320N2003
E1128O2:H42E23AMP CHL TET SSS STR CAZSame as donor1320N2002
E1216O33:H4E2AMP CHL GEN SXT TET SSS STR CAZAMP CHL SXT TET SSS CAZ1020Y2002
E1246n:H30E4AMP CHL GEN KAN SXT TET SSS STR CAZAMP CHL SXT TET SSS CAZ1320Y2002
E1291O159:H4E24AMP CHL TET SSS STR CAZSame as donor1020Y2001
E1445O35:H16E25AMP CHL KAN SXT TET SSS STR CAZSame as donor2420Y2001
E1455O145:+E26AMP CHL GEN KAN TET SSS STR CAZSame as donor233.2Y2001
E1473O35:H16E27AMP CHL KAN SXT TET SSS STR CAZSame as donor2420Y2001
E2275O66:H25E28AMP CHL GEN KAN SXT TET SSS STR CAZSame as donor13.2N2001
E2323O154:H30E13AMP CHL KAN SXT TET SSS STR CAZAMP CHL TET SSS CAZ163.2N2002
E2331n:H9E29AMP CHL TET SSS STR CAZSame as donor173.2N2002
E2346O154:H30E14AMP CHL KAN SXT TET SSS STR CAZAMP CHL TET SSS CAZ173.2N2002
E2358n:H42E30AMP CHL GEN KAN TET SSS STR CAZSame as donor233.2N2002
E2379n:H48E31AMP CHL GEN KAN TET SSS STR CAZSame as donor243.2N2002
E2449n:H42E32AMP CHL TET SSS STR CAZSame as donor1320Y2002
E2469O20:H30E33AMP CHL KAN SXT TET SSS STR CAZSame as donor2020Y2002
E2517n:H38E34AMP CHL GEN KAN SXT TET SSS STR CAZSame as donor53.2Y2002
E2530O111:H8E11AMP CHL KAN SXT TET SSS STR CAZAMP CHL SXT TET SSS CAZ261N2002
E2588n:H11E19AMP CHL GEN KAN SXT TET SSS STR CAZAMP CHL GEN KAN TET SSS CAZ1920N2002
E2639O8w:H16E18AMP CHL KAN TET SSS STR CAZAMP CAZ22.9N2002
E2654n:H38E35AMP CHL KAN SXT TET SSS STR CAZSame as donor2420N2002
E2676O11:H15E5AMP CHL GEN KAN SXT TET SSS STR CAZAMP CHL TET SSS CAZ163.2N2002
E2679n:H5or56E20AMP CHL GEN KAN SXT TET SSS STR CAZAMP CHL GEN KAN TET SSS CAZ213.2N2002
E2699n:H28E21AMP CHL GEN KAN SXT TET SSS STR CAZAMP CHL KAN SUL CAZ2220Y2002
E2713n:H4E15AMP CHL KAN SXT TET SSS STR CAZAMP CHL TET SSS CAZ1320N2002
E2759O20:H18E3AMP CHL GEN KAN SXT TET SSS STR CAZAMP CHL KAN SXT TET SSS CAZ2420Y2002
E2769n:H4E9AMP CHL KAN SXT TET SSS STR CAZAMP CHL SXT TET SSS CAZ183.2Y2002
E525O20:H30E22AMP CHL KAN SXT TET SSS STR CAZSame as donor2020Y2002
E6875O2:H42E16AMP CHL KAN SXT TET SSS STR CAZAMP CHL TET SSS CAZ173.2N2002
E6900a:5or56E12AMP CHL GEN KAN SXT TET SSS STR CAZAMP CHL GEN TET SSS CAZ153.2N2003
E7119O2:H42E17AMP CHL KAN SXT TET SSS STR CAZAMP CHL TET SSS CAZ153.2N2003
E7132O116w:H9E36AMP CHL SXT TET SSS STR CAZSame as donor1620N2003
E7140O116w:H9E37AMP CHL TET SSS STR CAZSame as donor811, ∼20N2003
E7196O101:H9E6AMP CHL GEN KAN SXT TET SSS STR CAZAMP CHL TET SSS CAZ1020Y2002
E7212n:H9E7AMP CHL GEN KAN TET SSS STR CAZAMP CHL TET SSS CAZ1320Y2003
E7292O117:H4E8AMP CHL GEN SXT TET SSS STR CAZAMP CHL TET SSS CAZ343.2N2003
E7403O145:H12E10AMP CHL GEN KAN TET SSS STR CAZAMP CHL GEN TET SSS CAZ73.2, 11N2003
E793O20:H30E1AMP CHL KAN SXT TET SSS STR CAZAMP CAZ63.2Y2003
E8100O10:H42E39AMP CHL TET SSS STR CAZSame as donor271, 3.2N2003
E8142n:H9E41AMP CHL SXT TET SSS STR CAZSame as donor1320Y2003
E8166n:H9E42AMP CHL SXT TET SSS STR CAZSame as donor1020N2003
E8188O159:H4E43AMP CHL TET SSS STR CAZSame as donor2620N2003
E8430O159:H4E44AMP CHL TET SSS STR CAZSame as donor2620N2003
E8431O159:H4E45AMP CHL TET SSS STR CAZSame as donor2820N2003
E8603n:nE46AMP CAZSame as donor42.9, 4, 5Y2003
E8618O159:H4E38AMP CHL TET SSS STR CAZSame as donor1220N2003
E8625O159:H4E40AMP CHL TET SSS STR CAZSame as donor2520N2003
  • a n, negative; + positive, novel antigen; a, autoagglutination; w, weak agglutination.

  • b AMP, ampicillin; CHL, chloramphenicol; GEN, gentamicin; KAN, kanamycin; SXT, sulfamethoxizone/trimethoprim; TET, tetracycline; STR, streptomycin; SSS, triple sulfa; CAZ, ceftazidime.

  • c “Same as donor” is with respect to all resistances except streptomycin, as the recipient strain (DH10B) is intrinsically resistant to streptomycin.

  • d A blaCMY-2-positive PstI fragment of 850 bp was present for all plasmids, in addition to the larger fragment reported in this column.

  • e N, no; Y, yes.

  • f Transconjugants were observed only when the helper plasmid pRK2013 was included in the mating mixture.