TABLE 3.

Relative expression of potential CpxR-regulated genes in the ΔcpxR1 mutant

ClassGeneTranscript level in ΔcpxR (fold increase compared with the wild type)a
OD600, ∼0.5OD600, ∼0.8OD600, ∼5.0Plate
Hemolysin relatedxaxA6.96 (5)b0.38 (3)1.17 (4)ND
xhlB1.71 (9)1.72 (2)0.41 (7)ND
Protease relatedprtA3.05 (5)dND0.58 (7)ND
Lipase relatedlrhA0.60 (9)0.29 (7)c0.77 (5)ND
xlpA1.06 (8)0.75 (5)1.05 (4)ND
Flagellum relatedfliA1.06 (3)1.51 (3)NDND
flhD1.57 (8)0.84 (6)0.77 (3)ND
flgE1.05 (9)1.21 (6)0.84 (6)ND
fliC1.06 (9)1.46 (6)0.61 (3)ND
Pilus relatedmrxANDNDND3.36 (5)b
  • a The numbers in parentheses are the numbers of replicates. ND, not determined.

  • b The value is significantly different from the wild-type value, which was defined as 1.0 (P < 0.01).

  • c The value is significantly different from the wild-type value, which was defined as 1.0 (P < 0.05).

  • d The value is significantly different from the wild-type value, which was defined as 1.0 (P < 0.001).