TABLE 1.

Strains and plasmids

Plasmid or strainDescriptionReference or source
Plasmids
    pIMKSite-specific listerial integrative vector, 5.1 kb, KanrThis study
    pIMK2Site-specific listerial integrative vector, constitutive overexpression, 6.2 kb, KanrThis study
    pIMK3Site-specific listerial integrative vector, high-level IPTG-controlled gene expression, 7.5 kb, KanrThis study
    pIMK4Site-specific listerial integrative vector, strictly IPTG-controlled gene expression, 7.5 kb, KanrThis study
    pPL2Site-specific listerial integrative vector, 6.1 kb, Cmr37
    pORI280RepA gene replacement vector, constitutive lacZ, 5.3 kb, Emr40
    pPL2lux-PhelpSite-specific listerial integrative vector, constitutive high-level luciferase expression, 11.8 kb, Cmr59
    pVE6007Temperature-sensitive helper plasmid, supplies RepA in trans47
    pNZ8048Lactococcal vector for nisin-inducible gene expression, 3 kb, Cmr48
    pIMK2luxPhelp-driven constitutive LuxABCDE overexpression, KanrThis study
    pIMK3luxIPTG-inducible high-level LuxABCDE expression, KanrThis study
    pIMK4luxIPTG-inducible strict LuxABCDE expression, KanrThis study
    pIMK2p60Phelp-driven constitutive P60 overexpression, KanrThis study
    pIMK3p60IPTG-inducible high-level P60 expression, KanrThis study
    pIMK4p60IPTG-inducible strict P60 expression, KanrThis study
    pIMK2inlAPhelp-driven constitutive InlA overexpression, KanrThis study
    pIMK3inlAIPTG-inducible high-level InlA expression, KanrThis study
    pIMK4inlAIPTG-inducible strict InlA expression, KanrThis study
    pIMK4secAIPTG-inducible strict SecA expression, KanrThis study
    phlyChlyA promoter fused to CAT gene, cloned into pIMK (AatII/NdeI)This study
E. coli strains
    XL1-BlueCloning host strainStratagene
    DH10BCloning host strainInvitrogen
    EC101JM109 derivative, Kanr and RepA integrated in the glgB gene38
    EC10BDH10B derivative, Kanr and RepA integrated in the glgB geneThis study
    EC10B/pORI280Δp60pORI280 containing 400-bp region on either side flanking p60 deletion (2 to 484 amino acids)This study
    EC10B/pORI280ΔinlApORI280 containing 400-bp region on either side flanking inlA deletion (80 to 506 amino acids)This study
    EC10B/pORI280prfA*pORI280 containing 400-bp region on either side flanking the mutated prfA nucleotideThis study
L. monocytogenes strains
    EGDeWild-type serotype 1/2a strain, genome sequenced27
    10403SWild-type serotype 1/2a strain, genome sequenced8
    F2365Wild-type serotype 4b strain, genome sequenced50
    EGDe::pIMK3EGDe transformed with vector only integrated at tRNAArg locusThis study
    EGDe::pIMK2luxEGDe with Lux overexpressed from the Phelp promoter integrated at tRNAArg locusThis study
    EGDe::pIMK3luxEGDe with Lux expressed from high-level IPTG-inducible promoter integrated at tRNAArg locusThis study
    EGDe::pIMK4luxEGDe with Lux expressed from low-level IPTG-inducible promoter integrated at tRNAArg locusThis study
    EGDeΔp60EGDe with the entire p60 gene deleted (amino acids 2 to 484)This study
    EGDe::pIMK2p60EGDe with P60 overexpressed from he Phelp promoter integrated at tRNAArg locusThis study
    EGDeΔp60::pIMK2p60EGDeΔp60 with P60 overexpressed from the Phelp promoter integrated at tRNAArg locusThis study
    EGDeΔp60::pIMK3p60EGDeΔp60 with P60 expressed from high-level IPTG-inducible promoter integrated at tRNAArg locusThis study
    EGDeΔp60::pIMK4p60EGDeΔp60 with P60 expressed from strict IPTG-inducible promoter integrated at tRNAArg locusThis study
    EGDeΔinlAEGDe with the E-cadherin interacting region of InlA deleted (amino acids 80 to 506)This study
    EGDeΔinlA::pIMK2inlAEGDeΔinlA with InlA overexpressed from the Phelp promoter integrated at tRNAArg locusThis study
    EGDeΔinlA::pIMK3inlAEGDeΔinlA with InlA expressed from high-level IPTG-inducible promoter integrated at tRNAArg locusThis study
    EGDeΔinlA::pIMK4inlAEGDeΔinlA with InlA expressed from a strict IPTG-inducible promoter integrated at tRNAArg locusThis study
    EGDeΔsecA::pIMK4secAEGDe with the entire secA gene deleted (amino acids 2 to 837), complemented with secA under the control of a strict IPTG-dependent promoter integrated at tRNAArg locusThis study