TABLE 3.

Quantitative estimates of destruxins A and B in crude broth and mycelial extracts of M. anisopliae ΔManps1 mutant (KO 8-18 and KO B1-3) and control (ARSEF 2575 and Ect A-18) strainsa

StrainMycelial dry wt (mean mg/culture ± SEM)Amt in broth (mean mg/liter ± SEM)Amt in mycelium (mean mg/liter ± SEM)
Destruxin ADestruxin BDestruxin ADestruxin B
ARSEF 2575742 ± 15 a72.7 ± 0.7 a37.7 ± 0.6 a4.5 ± 0.6 a2.8 ± 0.4 a
Ect A-18983 ± 63 a75.3 ± 16.4 a36.8 ± 3.1 a2.7 ± 1.0 a2.8 ± 0.3 a
KO 8-181038 ± 167 a62.5 ± 22.6 a36.6 ± 8.6 a8.5 ± 6.0 a4.7 ± 2.5 a
KO B1-3829 ± 87 a65.6 ± 9.6 a16.7 ± 1.5 b3.8 ± 0.9 a1.1 ± 0.2 ab
  • a Estimates are expressed as the mean of three replicated extractions from 11-day-old 100-ml cultures. Means within a column followed by the same letter are not significantly different at P < 0.05 in Tukey's HSD test.

  • b The amount of destruxin B produced by KO B1-3 in mycelium was determined to be significantly less than that of the two control strains only when KO 8-18, which was highly variable in this assay, was removed from the statistical analysis. See the text discussing the chemical analyses of the ΔManps1 mutants and control strains in Results for more details.