TABLE 4.

Mortality and survival times for larvae of the BAW, Spodoptera exigua, and CPB, Leptinotarsa decemlineata, inoculated with spores from the ΔManps1 mutant and control strains of M. anisopliaea

StrainbSpodoptera exiguaLeptinotarsa decemlineata
Avg % mortality (SEM)Mean survival time (SEM)No. of insectsAvg % mortality (SEM)Mean survival time (SEM)No. of insects
ARSEF 257586 (7)3.1 (0.05)28244 (9)4.5 (0.13)63
Ect A-1889 (7)3.1 (0.04)29243 (11)4.5 (0.23)32
KO 8-1889 (8)3.1 (0.05)19344 (9)4.3 (0.13)54
KO 37-289 (11)3.2 (0.06)22048 (8)4.6 (0.15)62
KO 43-195 (2)3.0 (0.04)22444 (11)4.5 (0.14)40
KO B1-3NTNTNT38 (9)4.8 (0.22)30
  • a For the percent mortality, no significant difference was observed among strains by analysis of variance. Analysis was done on arcsine-square root-transformed percentages. For S. exigua, F = 0.8, df = 4, 19, and P < 0.5; for L. decemlineata, F = 0.13. df = 5, 37, and P < 0.9). For the survival time (in days), no significant difference was observed in insect survival times among isolates. For S. exigua, F = 1.4, df = 4, 1,206, and P < 0.2; for L. decemlineata, F = 0.9, df = 5, 275, and P < 0.5. The number of insects refers to the number of insects dead from fungal infection within 7 days of inoculation. NT, not tested.

  • b Four (L. decemlineata) to six (S. exigua) assays were conducted with each strain, using 47 to 60 insects per strain per assay.