TABLE 3.

Flow cytometric analyses of different GFP- and GFP fusion-expressing derivatives of M. gryphiswaldense cultivated under different conditions

StrainCulture conditionsGrowth stageaMagnetism (Cmag)Avg fluorescenceCell proportion above threshold (%)
M. gryphiswaldense(pCL5)1% oxygen; 100-ml culture in 1-liter flask0.0951.718.18.5
10% oxygen; 100-ml culture in 1-liter flask0.1831.541.920.0
21% oxygen; 100-ml culture in 1-liter flask0.1380.052.320.4
21% oxygen; 100-ml culture in 500-ml flask0.2240.050.122
21% oxygen; 400-ml culture in 500-ml flask0.2201.742.619.7
21% oxygen; 4-liter culture in 5-liter flask0.4082.030.822.3
M. gryphiswaldense(pCL6)21% oxygen; 4-liter culture in 5-liter flask0.5321.94222
12 Pa oxygen; 10-liter fermentor0.6241.322.810.8
200 Pa oxygen; 10-liter fermentor0.5401.627.815.8
2 × 104 Pa oxygen; 10-liter fermentor0.4800.246.226.1
2 × 104 Pa oxygen for 16 h + 20 Pa for 8 h; 10-liter fermentor0.6241.342.420.3
M. gryphiswaldense(pCL7)21% oxygen; 4-liter culture in 5-liter flask0.4081.715.65.3
M. gryphiswaldense(pCL8)21% oxygen; 4-liter culture in 5-liter flask0.4161.524.917
  • a Optical density at 565 nm.