TABLE 2.

Downregulated proteins of A. fumigatus pkaC1Oex strain in comparison to the wild type after induction of pkaC1 expression

Putative function and spot no.aProtein nameLocus tag% Sequence coverageRegulation (fold)
Protein biosynthesis
    403Translation elongation factor EF-2 subunit, putativeAFUA_2G1353013.53.7
    441Translation elongation factor EF-2 subunit, putativeAFUA_2G1353019.45.6
    418Polyadenylate-binding proteinAFUA_1G0419050.52.4
    85240S ribosomal protein S0AFUA_3G1332050.58.1
Purine biosynthesis
    426Ade1p proteinAFUA_6G0473027.52.3
Pyrimidine/arginine biosynthesis
    275Carbamoyl-phosphate synthetase, large subunitAFUA_2G1007018.23.2
Tricarboxylic acid cycle
    1007Malate dehydrogenase, NAD dependentAFUA_6G0521071.53.0
Glyoxylate cycle
    565Malate synthaseAFUA_6G0354032.42.4
    628Malate synthaseAFUA_6G0354025.42.7
    634Malate synthaseAFUA_6G0354037.92.7
    2100Isocitrate lyase (AcuD)AFUA_6G0286017.42.4
Gluconeogenesis
    619Phosphoenolpyruvate carboxykinase (AcuF)AFUA_6G0772028.32.8
    2203Phosphoenolpyruvate carboxykinase (AcuF)AFUA_6G0772034.62.5
Pentose phosphate pathway
    1039Ribose 5-phosphate isomerase AAFUA_6G1061018.42.1
Acetate degradation
    501Acetyl-CoA synthetase (FacA)AFUA_4G1108016.03.5
    510Acetyl-CoA synthetase (FacA)AFUA_4G1108018.211.0
    518Acetyl-CoA synthetase (FacA)AFUA_4G1108026.72.8
    519Acetyl-CoA synthetase (FacA)AFUA_4G1108029.74.8
    2266Acetyl-CoA synthetase (FacA)AFUA_4G1108030.47.6
Alcohol degradation
    891Alcohol dehydrogenase (ADH1)AFUA_7G0101037.72.8
    914Alcohol dehydrogenase (ADH1)AFUA_7G0101033.72.6
Fatty acid degradation
    7913-Ketoacyl-CoA ketothiolase (Kat1), putativeAFUA_1G1265045.53.0
Propionate degradation
    771Methylcitrate synthaseAFUA_6G0359040.02.4
Electron transport and reduction
    835FlavohemoproteinAFUA_8G0608022.92.2
    2179Assimilatory sulfite reductaseAFUA_6G0892017.02.2
Cytoskeleton assembly
    701Tubulin beta-2 subunitAFUA_7G0025022.63.2
    710Tubulin beta-2 subunitAFUA_7G0025023.32.0
    780SeptinAFUA_5G0308021.62.7
Protection against oxidative stress
    464Mycelial catalase Cat1AFUA_3G0227034.62.1
    466Mycelial catalase Cat1AFUA_3G0227037.85.8
Siderophore biosynthesis
    656 l-Ornithine N-5-oxygenaseAFUA_2G0768045.52.7
Hemolysis
    1457Asp hemolysinAFUA_3G0059050.42.4
Others and proteins of unknown function
    597GMC oxidoreductaseAFUA_3G0158049.12.4
    603GMC oxidoreductaseAFUA_3G0158025.98.0
    610GMC oxidoreductaseAFUA_3G0158041.32.8
    545AMP-binding domain protein, putativeAFUA_1G0598038.33.6
    593Pyruvate decarboxylase PdcA, putativeAFUA_3G1107037.12.1
    611Pyridine nucleotide-disulfide oxidoreductase (Nfrl), putativeAFUA_7G0207037.13.2
    719Homocitrate synthaseAFUA_4G1046022.22.1
    739Gamma-butyrobetaine hydroxylase subfamily, putativeAFUA_2G1497043.32.6
    776Acyl-CoA dehydrogenase family proteinAFUA_5G0650024.62.2
    8342-Amino-3-carboxymuconate-6-semialdehyde decarboxylase, putativeAFUA_5G1246027.92.0
    903Ran/Spi1 binding proteinAFUA_5G1218045.72.5
    887Unknown functionAFUA_6G1185057.42.4
    1019Short chain dehydrogenase, putativeAFUA_4G0871059.63.6
    1021Unknown functionAFUA_8G0055063.17.9
    1103Outer mitochondrial membrane protein porinAFUA_4G0691033.02.4
    1141DlpA domain proteinAFUA_4G1094029.42.1
    1165Unknown functionAFUA_5G1468063.02.1
    1195Rho-GDP dissociation inhibitorAFUA_5G1138029.42.2
    1320HEX1AFUA_5G0883030.32.3
    2176PH domain proteinAFUA_4G1245020.98.3
  • a Some spots represent different isoforms of the same protein. Proteins were identified by peptide mass fingerprinting. Spot numbers are shown in Fig. 6.