TABLE 1.

Cell numbers of five selected samples from each mine tailing dump located in Botswana, Sweden, and Germany determined by different quantification methodsa

Organism typeMethodCell no. (log n/g dry wt) for location, zone, and depth (m)
Selebi-Phikwe, Botswana (oxidized zone)Kristineberg, SwedenFreiberg, Germany (oxidation zone)
Oxidized zoneUnoxidized zone (2.65 m)
6.58.59.511.517.52.152.242.32.390.630.640.650.680.77
TotalSybr green7.68.17.98.78.28.98.69.08.56.59.49.39.49.68.3
BacteriaQ-PCR7.17.58.18.16.77.77.17.83.83.98.18.48.88.48.9
CARD-FISH6.97.57.67.76.57.76.87.87.16.07.98.88.48.58.2
FISH5.86.4ND6.35.1ND6.3NDNDND8.18.98.48.68.4
ArchaeaQ-PCRNDNDNDNDND5.95.46.87.9NDNDNDNDNDND
Fe(II) oxidizersMPN5.57.17.17.15.45.53.82.86.8ND8.88.89.28.88.8
S oxidizersMPNNMNMNMNMNMNMNMNMNMNM6.86.26.57.87.8
Acidithiobacillus spp.Q-PCR3.63.64.03.7ND5.83.85.76.5ND7.17.17.57.27.4
FISHND5.8ND5.8NDNDNDNDNDND8.08.88.18.67.7
A. ferrooxidansFISHNDNDNDNDNDNDNDNDNDND7.78.88.48.57.4
A. caldusQ-PCRNDNDNDNDND4.23.6ND3.3NDNDNDNDNDND
Leptospirillum spp.Q-PCRNDNDNDNDND5.14.36.36.7ND5.84.55.4ND4.4
L. ferrooxidansFISHNDNDNDNDNDNDNDNDNDND5.9ND5.8NDND
Sulfobacillus spp.Q-PCRNDND4.6NDND4.23.24.54.9NDNDNDNDNDND
Acidiphilium spp.FISHND5.3ND5.6NDNDNDNDNDNDNDNDNDNDND
GeobacteraceaeQ-PCRND4.1ND5.14.15.0NDNDNDNDNDNDNDNDND
Sulfate reducers (dsr)Q-PCRND5.76.0NDND6.65.6ND6.14.2NDND6.15.1ND
  • a Cell numbers were below detection limits for FISH and CARD-FISH for Archaea, FISH for Acidimicrobium and relatives, FISH for gram-positive Bacteria with low G+C contents, and FISH for Sulfobacillus spp. in the investigated samples of all three sites. Conversion factors for calculating cell numbers from DNA copy numbers and detection limits of the methods are given in Materials and Methods. NM, not measured; ND, not detected.