Digital image analysis of nucleoids from E. colia

Dose (μg/ml)nSurface area (mean ± SD)Relative increase in surface areaP
01507,411.9 ± 2,173.8
0.01214529,198.8 ± 12,747.93.9<0.0001
5.014685,156.8 ± 19,301.111.5<0.0001
  • a Nucleoids were treated with ciprofloxacin for 40 min in LB broth and processed with the Micro-Halomax kit. n, number of nucleoids examined; P, significance level obtained with Mann-Whitney U test; surface area is shown in number of pixels.