TABLE 1.

Fungal DGGE-PCR primers (18S or ITS) used to detect unculturable fungi in the Hawaiian marine sponge Suberites zeteki

DGGE-PCR set IDPrimersDGGE-PCR productsSequenced DGGE-PCR clonesDGGE band no.Fungal taxon no.Reference(s)
Primary PCRaNested PCRSize (bp)TargetNo. of fungal insertsNo. of sponge inserts
1ITS1/ITS2-GC350ITS0135021
2ITS1F/ITS2-GC350ITS0107021
7NS1/GCFung40018S1403756
3P1-GC/P2350ITS1/5.8SNAbNA9NA25
4EF4/EF3EF4/NS3-GC50018SNANANANA11, 75
5EF4/fung5EF4/NS3-GC50018SNANANANA11, 75
8NS1/NS8NS1/NS2 + 10-GC60018S1612443
10ITS1F/ITS4ITS3/ITS4-GC450ITS461510234
  • a PCR cycling conditions are described in the cited references, as indicated in the text.

  • b NA, not applicable. Refer to the text for details.