TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsReference or source
Strains
    E. coli EC101Cloning host; repA+ Kanr30
    L. lactis strains
        NZ9000MG1363, nisin-inducible overexpression host; pepN::nisRK14
        NZ9000-pNZ-αamyNZ9000 containing pNZ-αamyThis study
        NZ9000-pNZ-pullNZ9000 containing pNZ-pullThis study
    B. breve strains
        UCC2003Isolate from nursling stool37
        UCC2003-apuBpORI19-apuB insertion mutant of UCC2003This study
    Plasmids
        pNZ8048Cmr; nisin-inducible translational fusion vector14
        pNZ-αamyCmr; pNZ8048 derivative containing translational fusion of α-amylase encoding DNA fragment to nisin-inducible promoterThis study
        pNZ-pullCmr; pNZ8048 derivative containing translational fusion of pullulanase encoding DNA fragment to nisin-inducible promoter on pNZ8048This study
        pTGB019Cmr; 6.5-kb derivative of pVE6007 harboring temperature-sensitive repA geneL. Steidler and S. Neirinck, unpublished results
        pORI19Emr RepA Ori+; cloning vector30
        pORI19-apuBInternal 1-kb fragment of apuB cloned in pORI19This study